Kaya Sabriye, Aslan İbrahim, Kıraç Ebru, Karaarslan Taner, Aslan Mutay
Department of Medical Biochemistry, Akdeniz University Medical Faculty, Antalya, Turkey.
Endocrinology Clinic, Antalya Research and Education Hospital, Antalya, Turkey.
Ann Clin Lab Sci. 2019 Mar;49(2):242-248.
Limited data are available on serum levels of different sphingomyelin (SM) and ceramide (CER) species in acne vulgaris (AV).
This study aimed to identify circulating levels of neutral sphingomyelinase activity (N-SMase), ceramide-1-phosphate (C1P), sphingosine-1-phosphate (S1P), C16-C24 SMs and C16-C24 CERs in AV patients and controls.
Serum was collected from 30 AV patients and 20 age, gender-matched control subjects. Serum levels of C16-C24 SMs and C16-C24 CERs were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Serum activity of N-SMase was assayed by standard kit methods, C1P and S1P levels were determined by enzyme-linked immunosorbent assay (ELISA).
A significant increase was observed in serum levels of C16 SM in patients with AV compared to controls. No significant difference was found in C18 and C24 SM levels between the two groups. Very-long-chain C24 CER was significantly decreased in AV patients compared to controls. Long chain C16-C20 CER levels showed no significant difference between AV patients and controls. A significant positive correlation was found between serum total cholesterol levels and all measured SMs and CERs in both the control and patient groups. Patients with AV had increased circulating levels of C16 SM, C1P and lower circulating levels of C24 CER compared to healthy controls, which may provide prognostic value for the disease.
Future studies are needed to understand the role of altered sphingolipid levels in the pathophysiology of AV.
关于寻常痤疮（AV）患者血清中不同鞘磷脂（SM）和神经酰胺（CER）种类水平的数据有限。
本研究旨在确定寻常痤疮患者和对照组中中性鞘磷脂酶活性（N-SMase）、神经酰胺-1-磷酸（C1P）、鞘氨醇-1-磷酸（S1P）、C16 - C24 SMs和C16 - C24 CERs的循环水平。
收集30例寻常痤疮患者和20例年龄、性别匹配的对照受试者的血清。采用超快速液相色谱（UFLC）结合串联质谱（MS/MS）的优化多反应监测（MRM）方法测定血清中C16 - C24 SMs和C16 - C24 CERs的水平。采用标准试剂盒方法检测N-SMase的血清活性，采用酶联免疫吸附测定（ELISA）法测定C1P和S1P水平。
与对照组相比，寻常痤疮患者血清中C16 SM水平显著升高。两组间C18和C24 SM水平无显著差异。与对照组相比，寻常痤疮患者中极长链C24 CER显著降低。寻常痤疮患者和对照组之间长链C16 - C20 CER水平无显著差异。在对照组和患者组中，血清总胆固醇水平与所有检测的SMs和CERs之间均存在显著正相关。与健康对照组相比，寻常痤疮患者循环中C16 SM、C1P水平升高，C24 CER水平降低，这可能为该疾病提供预后价值。
需要进一步研究以了解鞘脂水平改变在寻常痤疮病理生理学中的作用。
