Welsch C W, O'Connor D H, Aylsworth C F, Sheffield L G
J Natl Cancer Inst. 1987 Mar;78(3):557-65.
Transplantation success rates of primary 7,12-dimethylbenz(a)anthracene [(DMBA) CAS: 57-97-6]-induced rat mammary carcinomas and normal rat mammary glandular epithelium into female athymic mice were compared. The rat mammary carcinomas obtained from female Sprague-Dawley rats were transplanted into host athymic mice (6-8 wk of age) as 1 x 1-cm slices xenografted sc (2 slices/mouse) or as enzymatically dissociated cells inoculated into the gland-free mammary fat pad. Normal rat mammary glands (No. 4 glands and 3- to 5-mo-old virgin rats) were transplanted into host athymic mice as whole, intact mammary glands sc (1 gland/mouse) or as enzymatically dissociated cells inoculated into the gland-free mammary fat pad. All (100%) of the normal rat mammary glands were readily accepted and maintained in the athymic mice when transplanted either sc as whole glands or as dispersed cells inoculated into the gland-free fat pad. In contrast, only 13-14% of the DMBA-induced rat mammary carcinomas were accepted and maintained in the athymic mice (transplanted as slices sc or as dispersed cells inoculated into the gland-free fat pad). Treatment of host athymic nude mice with an intense mammotropic hormonal stimulus (prolactin and/or ovarian steroids) markedly enhanced the developmental growth of the transplanted normal rat mammae (subcutaneous slices and fat-pad inoculates); such a hormonal stimulus did not influence the transplantation success rate of the DMBA-induced rat mammary carcinomas. Thus female athymic nude mice can readily accept and maintain transplants of normal rat mammae but not carcinogen-induced carcinomatous rat mammae; the meager acceptance rate of the carcinomatous rat mammae by the athymic nude mouse was not enhanced by providing the host mice with a potent mammotropic hormonal growth stimulant.
比较了原发性7,12-二甲基苯并(a)蒽[(DMBA)，化学物质登记号：57-97-6]诱导的大鼠乳腺癌和正常大鼠乳腺上皮移植到雌性无胸腺小鼠体内的成功率。从雌性斯普拉格-道利大鼠获得的大鼠乳腺癌，作为1×1厘米的切片皮下移植（每只小鼠2片），或作为酶解细胞接种到无腺体的乳腺脂肪垫，移植到宿主无胸腺小鼠（6-8周龄）体内。正常大鼠乳腺（第4号乳腺以及3至5月龄的处女大鼠）作为完整的乳腺皮下移植（每只小鼠1个乳腺），或作为酶解细胞接种到无腺体的乳腺脂肪垫，移植到宿主无胸腺小鼠体内。当正常大鼠乳腺作为完整腺体皮下移植或作为分散细胞接种到无腺体脂肪垫时，所有（100%）都能很容易地被无胸腺小鼠接受并维持。相比之下，只有13%-14%的DMBA诱导的大鼠乳腺癌能被无胸腺小鼠接受并维持（作为切片皮下移植或作为分散细胞接种到无腺体脂肪垫）。用强烈的促乳腺激素刺激（催乳素和/或卵巢类固醇）处理宿主无胸腺裸鼠，可显著促进移植的正常大鼠乳腺（皮下切片和脂肪垫接种物）的发育生长；这种激素刺激并不影响DMBA诱导的大鼠乳腺癌的移植成功率。因此，雌性无胸腺裸鼠能很容易地接受并维持正常大鼠乳腺的移植，但不能接受致癌物诱导的大鼠癌性乳腺的移植；给宿主小鼠提供强效的促乳腺激素生长刺激剂，并不会提高无胸腺裸鼠对癌性大鼠乳腺的低接受率。
