Department of Infectious Diseases and Clinical Microbiology, Gazi University School of Medicine, 06560, Ankara, Turkey.
Department of Clinical Microbiology, Gazi University School of Medicine, 06560, Ankara, Turkey.
J Antibiot (Tokyo). 2019 Aug;72(8):600-604. doi: 10.1038/s41429-019-0188-6. Epub 2019 Apr 26.
The synergistic activity of eravacycline in combination with colistin on carbapenem-resistant A. baumannii (CRAB) isolates was evaluated in this study. Minimum inhibitory concentrations (MICs) of eravacycline and colistin were determined by the broth microdilution method. MICs values ranged between 1 to 4 mg and 0.5 to 256 mg l for eravacycline and colistin, respectively. In vitro synergy between eravacycline and colistin was evaluated by using the chequerboard methodology. Synergistic activity was found in 10% of the strains, and additive effect in 30%. No antagonism was detected. Similar activity was also observed in colistin-resistant CRAB isolates. The result of this study indicates that eravacycline and colistin combination may be a potential therapeutic option for the treatment of CRAB related infections.
本研究评估了依拉环素与黏菌素联合应用对碳青霉烯类耐药鲍曼不动杆菌（CRAB）分离株的协同活性。采用肉汤微量稀释法测定依拉环素和黏菌素的最小抑菌浓度（MIC）。依拉环素和黏菌素的 MIC 值范围分别为 1 至 4mg 和 0.5 至 256mg/L。采用棋盘微量稀释法评估依拉环素和黏菌素之间的体外协同作用。在 10%的菌株中发现协同作用，在 30%的菌株中发现相加作用。未检测到拮抗作用。在黏菌素耐药的 CRAB 分离株中也观察到了类似的活性。本研究结果表明，依拉环素与黏菌素联合应用可能是治疗 CRAB 相关感染的一种潜在治疗选择。
