Gonzalez-Pena Veronica, Gawad Charles
Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Department of Computational Biology, St. Jude Children's Research Hospital, Memphis, TN, USA.
Methods Mol Biol. 2019;1979:227-234. doi: 10.1007/978-1-4939-9240-9_14.
Single-cell genome sequencing can detect low-frequency genetic alterations present in complex tissues. However, the experimental procedures are technically challenging. This includes dissociation of the tissue, isolation of single cells, whole-genome amplification, sequencing library preparation, and an optional target enrichment. Here we describe how to perform each of these processes to obtain high-quality single-cell genome sequencing data.
单细胞基因组测序能够检测复杂组织中存在的低频基因改变。然而，实验步骤在技术上具有挑战性。这包括组织解离、单细胞分离、全基因组扩增、测序文库制备以及可选的目标富集。在此，我们描述如何执行这些过程中的每一个以获得高质量的单细胞基因组测序数据。
