单细胞5-羟甲基胞嘧啶测序

Single-Cell 5fC Sequencing.

作者信息

Zhu Chenxu, Gao Yun, Peng Jinying, Tang Fuchou, Yi Chengqi

机构信息

State Key Laboratory of Protein and Plant Gene Research, School of Life Sciences, Peking University, Beijing, People's Republic of China.

Biodynamic Optical Imaging Center, Beijing Advanced Innovation Center for Genomics, School of Life Sciences, Peking University, Beijing, People's Republic of China.

出版信息

Methods Mol Biol. 2019;1979:251-267. doi: 10.1007/978-1-4939-9240-9_16.

DOI:10.1007/978-1-4939-9240-9_16

PMID:31028643

Abstract

Active DNA demethylation plays important roles in the epigenetic reprogramming of developmental processes. 5-formylcytosine (5fC) is produced during active demethylation of 5-methylcytosine (5mC). Here, we describe a technique called CLEVER-seq (Chemical-labeling-enabled C-to-T conversion sequencing), which detects the whole genome 5fC distribution at single-base and single-cell resolution. CLEVER-seq is suitable for the analysis of precious samples such as early embryos and laser microdissection captured samples.

摘要

活性DNA去甲基化在发育过程的表观遗传重编程中发挥着重要作用。5-甲基胞嘧啶（5mC）的活性去甲基化过程中会产生5-甲酰基胞嘧啶（5fC）。在此，我们描述了一种名为CLEVER-seq（化学标记辅助的C到T转换测序）的技术，该技术能够以单碱基和单细胞分辨率检测全基因组5fC分布。CLEVER-seq适用于分析珍贵样本，如早期胚胎和激光显微切割捕获的样本。