Bai Shiyan, Han Xiao, Feng Dan
College of Biological Science and Engineering, Fuzhou University, Fujian, China.
Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Plant Sci. 2023 Feb 20;14:1139744. doi: 10.3389/fpls.2023.1139744. eCollection 2023.
High concentrations of heavy metals in the environment will cause serious harm to ecosystems and human health. It is urgent to develop effective methods to control soil heavy metal pollution. Phytoremediation has advantages and potential for soil heavy metal pollution control. However, the current hyperaccumulators have the disadvantages of poor environmental adaptability, single enrichment species and small biomass. Based on the concept of modularity, synthetic biology makes it possible to design a wide range of organisms. In this paper, a comprehensive strategy of "microbial biosensor detection - phytoremediation - heavy metal recovery" for soil heavy metal pollution control was proposed, and the required steps were modified by using synthetic biology methods. This paper summarizes the new experimental methods that promote the discovery of synthetic biological elements and the construction of circuits, and combs the methods of producing transgenic plants to facilitate the transformation of constructed synthetic biological vectors. Finally, the problems that should be paid more attention to in the remediation of soil heavy metal pollution based on synthetic biology were discussed.
环境中高浓度的重金属会对生态系统和人类健康造成严重危害。开发有效的土壤重金属污染控制方法迫在眉睫。植物修复在土壤重金属污染控制方面具有优势和潜力。然而,目前的超富集植物存在环境适应性差、富集物种单一和生物量小等缺点。基于模块化概念,合成生物学使设计多种生物体成为可能。本文提出了一种“微生物生物传感器检测 - 植物修复 - 重金属回收”的土壤重金属污染控制综合策略,并利用合成生物学方法对所需步骤进行了改进。本文总结了促进合成生物学元件发现和电路构建的新实验方法,并梳理了生产转基因植物的方法,以促进构建的合成生物学载体的转化。最后,讨论了基于合成生物学的土壤重金属污染修复中应更多关注的问题。