单细胞中的CRISPR筛选

CRISPR Screening in Single Cells.

作者信息

Henriksson Johan

机构信息

Molecular Infection Medicine Sweden, Umeå University, Umeå, Sweden.

出版信息

Methods Mol Biol. 2019;1979:395-406. doi: 10.1007/978-1-4939-9240-9_23.

DOI:10.1007/978-1-4939-9240-9_23

PMID:31028650

Abstract

The combination of single-cell RNA-seq and CRISPR allows for efficient interrogation of possibly any number of genes, only limited by the sequencing capability. Here we describe the current protocols for CRISPR screening in single cells, from cloning and virus production to generating sequencing data.

摘要

单细胞RNA测序与CRISPR技术的结合能够对可能任意数量的基因进行高效检测，唯一的限制因素是测序能力。在此，我们描述了单细胞中CRISPR筛选的现行方案，从克隆、病毒生产到生成测序数据。