State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, PR China.
Biosens Bioelectron. 2019 Jul 1;136:31-37. doi: 10.1016/j.bios.2019.04.031. Epub 2019 Apr 19.
DNA-based nanomachines have received increasing attention due to their great potential to mimic natural biological motors and create novel modes of motion. Here, we report a DNAzyme-based walking machine, which can operate in living cells after triggered by intracellular miRNA-21. The walking machine is constructed by assembling DNAzyme walking strands and FAM-labeled substrate strands on a single gold nanoparticle (AuNP). The DNAzyme walking strand is first silenced by a blocker strand. After cellular uptake, DNAzyme-based walker can be triggered by intracellular miRNA-21 and autonomously walk along the AuNP-based 3D track fueled by DNAzyme-catalyzed substrate cleavage. Each walking step results in the cleavage of a substrate strand and the release of a FAM-labeled DNA strand, allowing real-time monitoring of the operation of the machine. The DNAzyme-based walking machine has been successfully applied to image and monitor miRNA-21 expression levels in living cells with excellent specificity and reliability. This walking machine would hold great potential in the miRNA associated biological research and disease diagnostics.
基于 DNA 的纳米机器由于其模仿天然生物马达和创造新型运动模式的巨大潜力而受到越来越多的关注。在这里，我们报告了一种基于 DNA 酶的步行机器，它可以在被细胞内的 microRNA-21 触发后在活细胞中运行。该步行机是通过将 DNA 酶行走链和 FAM 标记的底物链组装在单个金纳米粒子 (AuNP) 上构建而成的。DNA 酶行走链首先被阻断链沉默。细胞摄取后，基于 DNA 酶的 walker 可以被细胞内的 microRNA-21 触发，并在 DNA 酶催化的底物切割提供动力的基于 AuNP 的 3D 轨道上自主行走。每次行走都会导致一个底物链的切割和一个 FAM 标记的 DNA 链的释放，从而可以实时监测机器的运行情况。基于 DNA 酶的步行机已成功应用于活细胞中 microRNA-21 表达水平的成像和监测，具有优异的特异性和可靠性。这种步行机在与 microRNA 相关的生物研究和疾病诊断方面具有很大的潜力。
