Henderson D S, Bailey D A, Sinclair D A, Grigliatti T A
Mutat Res. 1987 Mar;177(1):83-93. doi: 10.1016/0027-5107(87)90024-8.
We have undertaken the study of a collection of 32 Drosophila melanogaster mus strains selected on the basis of developmental sensitivity to the DNA-damaging agents, methyl methanesulfonate (MMS), N-acetyl-2-aminofluorene (AAF), nitrogen mustard (HN2), and gamma-radiation. In total, 18 of these strains are sensitive to MMS. In turn, 14 of these exhibit unconditional MMS sensitivity (one of the latter mutants is lethal at 29 degrees C), whereas the other 4 are sensitive to MMS only at higher temperatures. Detailed analysis of the 7 strongest MMS-sensitive strains reveals that they identify 4 new second chromosome mus loci. Two mus loci are each represented by two alleles. One mutant (mus205B1) is allelic to a previously characterized mus locus. Different MMS-sensitive mutants display patterns of mutagen cross-sensitivity (to AAF, HN2, benzo[a]pyrene (BP), and gamma-rays) that parallel the range of responses seen in previously recovered X-linked and autosomal mus loci. In general, mutations that are strongly sensitive to MMS are also sensitive to one or both of the procarcinogens, AAF and BP, as opposed to HN2 and gamma-radiation. In contrast, the moderately MMS-sensitive mutations are sensitive to HN2 and gamma-rays, but not to AAF or BP. Of the 14 mus strains that are not sensitive to MMS, 5 are sensitive to AAF, another 5 are sensitive to HN2, and the remaining 4 are sensitive to gamma-rays.
我们对一组32个黑腹果蝇mus菌株进行了研究，这些菌株是根据对DNA损伤剂甲磺酸甲酯（MMS）、N-乙酰-2-氨基芴（AAF）、氮芥（HN2）和γ射线的发育敏感性选择出来的。这些菌株中总共有18个对MMS敏感。其中，14个表现出无条件的MMS敏感性（后一种突变体中有一个在29摄氏度时致死），而另外4个仅在较高温度下对MMS敏感。对7个最强的MMS敏感菌株的详细分析表明，它们确定了4个新的第二染色体mus位点。两个mus位点各由两个等位基因代表。一个突变体（mus205B1）与一个先前已鉴定的mus位点等位。不同的MMS敏感突变体表现出诱变交叉敏感性模式（对AAF、HN2、苯并[a]芘（BP）和γ射线），这与先前在X连锁和常染色体mus位点中观察到的反应范围相似。一般来说，对MMS高度敏感的突变对一种或两种前致癌物AAF和BP也敏感，而对HN2和γ射线不敏感。相比之下，对MMS中度敏感的突变对HN2和γ射线敏感，但对AAF或BP不敏感。在14个对MMS不敏感的mus菌株中，5个对AAF敏感，另外5个对HN2敏感，其余4个对γ射线敏感。
