解码和分析印度茶（Camellia assamica）细胞器基因组以进行系统发育确认。
Decoding and analysis of organelle genomes of Indian tea (Camellia assamica) for phylogenetic confirmation.
机构信息
ICAR-National Research Centre on Plant Biotechnology, Pusa, New Delhi 110012, India.
Tea Board, Ministry of Commerce and Industry, Govt. of India, 14, B.T.M. Sarani, Kolkata 700 001, India.
出版信息
Genomics. 2020 Jan;112(1):659-668. doi: 10.1016/j.ygeno.2019.04.018. Epub 2019 Apr 25.
The NCBI database has >15 chloroplast (cp) genome sequences available for different Camellia species but none for C. assamica. There is no report of any mitochondrial (mt) genome in the Camellia genus or Theaceae family. With the strong believes that these organelle genomes can play a great tool for taxonomic and phylogenetic analysis, we successfully assembled and analyzed cp and mt genome of C. assamica. We assembled the complete mt genome of C. assamica in a single circular contig of 707,441 bp length comprising of a total of 66 annotated genes, including 35 protein-coding genes, 29 tRNAs and two rRNAs. The first ever cp genome of C. assamica resulted in a circular contig of 157,353 bp length with a typical quadripartite structure. Phylogenetic analysis based on these organelle genomes showed that C. assamica was closely related to C. sinensis and C. leptophylla. It also supports Caryophyllales as Superasterids.
NCBI 数据库提供了超过 15 个不同茶树物种的叶绿体 (cp) 基因组序列，但没有关于 C. assamica 的序列。在山茶属或茶科家族中，也没有线粒体 (mt) 基因组的报道。由于坚信这些细胞器基因组可以成为分类学和系统发育分析的有力工具，我们成功地组装和分析了 C. assamica 的 cp 和 mt 基因组。我们组装了 C. assamica 的完整 mt 基因组，它是一个长 707,441 bp 的单环连续序列，共包含 66 个注释基因，包括 35 个蛋白质编码基因、29 个 tRNA 和两个 rRNA。这是首次对 C. assamica 的 cp 基因组进行组装，得到了一个长 157,353 bp 的环形连续序列，具有典型的四分体结构。基于这些细胞器基因组的系统发育分析表明，C. assamica 与 C. sinensis 和 C. leptophylla 关系密切。它也支持石竹目作为超级类石松目。
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