基于汞（II）对谷胱甘肽稳定的四苯基卟啉修饰金纳米簇荧光猝灭效应的比率荧光法测定汞（II）。
Ratiometric fluorometric determination of mercury(II) by exploiting its quenching effect on glutathione-stabilized and tetraphenylporphyrin modified gold nanoclusters.
机构信息
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.
College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, 710062, China.
出版信息
Mikrochim Acta. 2019 Apr 27;186(5):307. doi: 10.1007/s00604-019-3405-x.
A ratiometric fluorometric assay for mercury(II) ion is described. It is making use of glutathione-stabilized gold nanoclusters (GSH-AuNCs) modified with tetraphenylporphyrin tetrasulfonic acid (TPPS). The resultant GSH-AuNC/TPPS nanocomposite displays dual emission (at 572 and 664 nm) under a single excitation wavelength of 365 nm. Mercury(II) ion intensively quenches the yellow fluorescence of GSH-AuNCs (peaking at 572 nm) but has a negligible effect on the red fluorescence of TPPS (at 664 nm). The ratio of fluorescence intensities at 572 and 664 nm drops linearly with Hg(II) ion concentration in the 0.02-2.0 μmol·L range, and the detection limit is 7 nmol·L (3s/S). The relative standard deviation (RSD) of the assay is 2.0% at a 0.5 μmol·L concentration level (n = 11). The method was successfully applied to the determination of Hg(II) ion in spiked water samples, with recoveries within the range of 87.5-107.5%. Graphical abstract Ratiometric fluorescence detection of mercury(II).
一种用于汞(II)离子的比率荧光分析方法。该方法利用谷胱甘肽稳定的金纳米簇（GSH-AuNCs）修饰四苯基卟啉四磺酸（TPPS）。所得的 GSH-AuNC/TPPS 纳米复合材料在 365nm 的单一激发波长下显示出双重发射（在 572nm 和 664nm 处）。汞（II）离子强烈猝灭 GSH-AuNCs 的黄色荧光（在 572nm 处达到峰值），但对 TPPS 的红色荧光（在 664nm 处）几乎没有影响。572nm 和 664nm 处荧光强度的比值随 Hg(II)离子浓度在 0.02-2.0μmol·L 范围内呈线性下降，检测限为 7nmol·L（3s/S）。在 0.5μmol·L 浓度水平下，该测定方法的相对标准偏差（RSD）为 2.0%（n=11）。该方法成功应用于加标水样中 Hg(II)离子的测定，回收率在 87.5-107.5%范围内。
Zotero 插件