Taghibakhsh Mehrdad, Farhadi Sareh, Babaee Afsaneh, Sheikhi Maryam
Department of Oral Medicine, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University,Tehran, Iran. Email:
Asian Pac J Cancer Prev. 2019 Apr 29;20(4):1109-1112. doi: 10.31557/APJCP.2019.20.4.1109.
Background and aim: Cigarettes, hookah, and tobacco are the most important etiologic factors for oral cancers and dysplastic lesions. This study was undertaken to determine the correlation between hookah use and the percentage of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa; and secondly to compare hookah user and non-user in terms of repair index. Materials and methods: The present historical cohort study was carried out on 72 samples taken from 36 hookah users and 36 control subjects. Smear samples were obtained from participants’ buccal mucosa for cytological evaluation using Papanicolaou technique. Then, the percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg were recorded and the repair index was calculated. Data were analyzed using Mann-Whitney U test. Results: A total of 72 samples taken from 36 hookah users and 36 control subjects were evaluated. The means of micronucleus scores in the buccal mucosa cells of hookah users and controls were 10.7±2.6 and 5.8±2.0, the karyorrhexis scores in the hookah users and controls were 0.1±0.06 and 0.04±0.06, and the karyolysis scores in hookah users and controls were 0.16±0.05 and 0.08±0.06, respectively. These differences were statistically significant between hookah users and controls (P<0.001). The broken egg score was 0.66±0.07 for the hookah users and 0.03±0.04 for the control group, revealing a statistically significant difference (P<0.036). Finally, the repair index values were 0.03±0.01 and 0.05±0.13 in hookah users and controls, respectively. This difference was also significant (P<0.026). Conclusion: The percentages of cells with micronucleus, karyorrhexis, karyolysis, and broken egg in the buccal mucosa of hookah users were significantly higher than those in control group; in addition, the repair index of the buccal mucosa cells in hookah users was significantly lower than that in the control group.
香烟、水烟和烟草是口腔癌及发育异常性病变最重要的病因。本研究旨在确定水烟吸食与颊黏膜中出现微核、核固缩、核溶解及“破卵”细胞百分比之间的相关性；其次，比较水烟吸食者与非吸食者的修复指数。材料与方法：本历史性队列研究对取自36名水烟吸食者和36名对照者的72份样本进行。使用巴氏染色技术从参与者的颊黏膜获取涂片样本用于细胞学评估。然后，记录出现微核、核固缩、核溶解及“破卵”细胞的百分比，并计算修复指数。数据采用曼-惠特尼U检验进行分析。结果：共评估了取自36名水烟吸食者和36名对照者的72份样本。水烟吸食者和对照者颊黏膜细胞的微核评分均值分别为10.7±2.6和5.8±2.0，核固缩评分分别为0.1±0.06和0.04±0.06，核溶解评分分别为0.16±0.05和0.08±0.06。水烟吸食者与对照者之间这些差异具有统计学意义（P<0.001）。“破卵”评分水烟吸食者为0.66±0.07，对照组为0.03±0.04，显示出统计学显著差异（P<0.036）。最后，水烟吸食者和对照者的修复指数值分别为0.03±0.01和0.05±0.13。这一差异也具有显著性（P<0.026）。结论：水烟吸食者颊黏膜中出现微核、核固缩、核溶解及“破卵”细胞的百分比显著高于对照组；此外，水烟吸食者颊黏膜细胞的修复指数显著低于对照组。
