Garrido-Jimenez Sergio, Roman Angel-Carlos, Carvajal-Gonzalez Jose Maria
Departamento de Bioquímica, Biología Molecular y Genética, Facultad de Ciencias, Universidad de Extremadura, Badajoz, Spain.
Champalimaud Neuroscience Programme, Lisbon, Portugal.
Front Genet. 2019 Apr 12;10:328. doi: 10.3389/fgene.2019.00328. eCollection 2019.
Proper ciliary basal body positioning within a cell is key for cilia functioning. Centriole and basal body positioning depends on signaling pathways such as the planar cell polarity pathway (PCP) governed by Frizzled (Fz-PCP). There have been described two PCP pathways controlled by different protein complexes, the Frizzled-PCP and the Fat-PCP pathway. Centriole planar polarization in non-dividing cells is a dynamic process that depends on the Fz-PCP pathway to properly occur during development from flies to humans. However, the function of the Ft-PCP pathway in centrioles polarization is elusive. Here, we present a descriptive initial analysis of centrioles polarization in Fat-PCP loss of function (LOF) conditions. We found that Fat (Ft) and Dachsous (Ds) LOF showed a marked centrioles polarization defect similar to what we have previously reported in Fz-PCP alterations. Altogether, our data suggest that centriole planar polarization in Drosophila wings depends on both Ft-PCP and Fz-PCP pathways. Further analyses in single and double mutant conditions will be required to address the functional connection between PCP and centriole polarization in flies.
纤毛基体在细胞内的正确定位是纤毛发挥功能的关键。中心粒和基体的定位依赖于诸如由卷曲蛋白（Fz-PCP）调控的平面细胞极性通路（PCP）等信号通路。已经描述了由不同蛋白质复合物控制的两条PCP通路，即卷曲蛋白-PCP通路和Fat-PCP通路。非分裂细胞中的中心粒平面极化是一个动态过程，在从果蝇到人类的发育过程中，它依赖于Fz-PCP通路才能正常发生。然而，Ft-PCP通路在中心粒极化中的功能尚不清楚。在此，我们对Fat-PCP功能丧失（LOF）条件下的中心粒极化进行了描述性初步分析。我们发现，Fat（Ft）和Dachsous（Ds）功能丧失表现出明显的中心粒极化缺陷，类似于我们之前报道的Fz-PCP改变的情况。总之，我们的数据表明，果蝇翅膀中的中心粒平面极化依赖于Ft-PCP和Fz-PCP通路。需要在单突变和双突变条件下进行进一步分析，以探讨果蝇中PCP与中心粒极化之间的功能联系。
