Deep eutectic solvent for an expeditious sono-synthesis of novel series of -quinazolin-4-one derivatives as potential anti-cancer agents.

To produce a new category of anti-cancer compounds, a facile and environmentally sustainable method for preparing diversified -quinazolinones was demonstrated using recyclable deep eutectic solvent (DES) under ultrasonic irradiation. The reactions were performed smoothly with a wide scope of substrates affording the desired derivatives in good-to-excellent yields under an atom-economical pathway. Particularly, halogen substituents that are amenable for further synthetic elaborations are well tolerated. Furthermore, the 'greenness' of the protocol was assessed within the scope of several green metrics and found to display an excellent score in the specified parameters. Cytotoxic activity of all novel -quinazolinones was investigated utilizing two cancer cell lines: breast (MCF-7) and lung (A549) cell lines and their IC values were determined. Most of the prepared derivatives displayed fascinating inhibitory activity with IC values in a low micromolar range. Remarkably, the derivative [3,3'-(sulfonyl(4,1-phenylene))(2-methyl-6-nitroquinazolin-4(3)-one)] showed superior potency against MCF-7 and A549 cancer cell lines, with IC values of 1.26 µM and 2.75 µM, respectively. Moreover, this derivative was found to have low toxicity to the normal breast cell line (MCF-10A) and could serve as a promising lead candidate for further development.