Benny A G, Scott R H, Woodfield D G
Transfusion. 1987 Mar-Apr;27(2):174-7. doi: 10.1046/j.1537-2995.1987.27287150194.x.
Four years' experience with a method for preparing a high-purity, low-fibrinogen, heat-treated factor VIII concentrate is reported. The process, batch adsorption of a cryoprecipitate extract with controlled-pore glass granules, removes 77 percent of the cryoprecipitate fibrinogen, resulting in a final concentrate-specific activity of 0.74 IU factor VIII per mg of protein at a yield of 194 IU factor VIII per kg of starting plasma. Heat treatment of the lyophilized concentrate for 72 hours at 60 degrees C results in less than 10 percent loss of factor VIII activity. This process does not require expensive fractionation equipment, is suitable for small-to medium-scale batch concentrate production and could be adopted by moderately well-equipped regional blood processing laboratories for the decentralized production of a high-quality, heat-treated factor VIII concentrate.
本文报道了一种制备高纯度、低纤维蛋白原、经热处理的凝血因子 VIII 浓缩物方法的四年经验。该工艺是用可控孔径玻璃颗粒对冷沉淀提取物进行批量吸附，可去除 77%的冷沉淀纤维蛋白原，最终浓缩物的比活性为每毫克蛋白质 0.74 国际单位凝血因子 VIII，每千克起始血浆的产量为 194 国际单位凝血因子 VIII。将冻干浓缩物在 60℃下热处理 72 小时，凝血因子 VIII 活性损失不到 10%。此工艺不需要昂贵的分级分离设备，适用于中小规模的批量浓缩物生产，装备中等的地区血液处理实验室可采用该工艺分散生产高质量的经热处理的凝血因子 VIII 浓缩物。
