Margolis J, Rhoades P
Vox Sang. 1979;36(6):369-74. doi: 10.1111/j.1423-0410.1979.tb04514.x.
Cryoprecipitate prepared by a rapid thawing technique was pooled in batches of 600--720 donor units and washed with ice-cold Tris-citrate-NaCl solution. After dissolving at 37 degrees C, it was adsorbed with Al(OH)3 and kaolin, and cleared by centrifugation. The supernatant, diluted with 5% dextrose was passed repeatedly through a bed of Celite, filtered through a 293 mm X 0.3 micrometer membrane disc and lyophilized. Typical composition was 15 U . ml-1 factor VII, and 40 mg . ml-1 protein with a yield of 300 U/l of starting plasma. The crude factor VIII concentrate was also a suitable material for preparation of high-purity factor VIII by controlled pore glass chromatography.
采用快速解冻技术制备的冷沉淀，按600 - 720个供体单位一批进行汇集，并用冰冷的柠檬酸三钠 - 氯化钠溶液洗涤。在37℃溶解后，用氢氧化铝和高岭土吸附，然后离心澄清。上清液用5%葡萄糖稀释后，反复通过硅藻土柱，经293mm×0.3微米的膜盘过滤并冻干。典型成分是每毫升含15单位的凝血因子VII和每毫升40毫克蛋白质，起始血浆的产率为每升300单位。粗制的凝血因子VIII浓缩物也是通过可控孔径玻璃色谱法制备高纯度凝血因子VIII的合适材料。
