Department of Periodontology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Peking University School and Hospital of Stomatology, Beijing, China.
Third Clinical Division, Peking University School and Hospital of Stomatology, Beijing, China.
J Periodontal Res. 2019 Oct;54(5):546-554. doi: 10.1111/jre.12658. Epub 2019 Apr 29.
CYP1A1 rs1048943 polymorphism was reported to be correlated with periodontitis; however, its association with aggressive periodontitis (AgP) has not yet been investigated. The aim of the study was to investigate the association between the CYP1A1 gene rs1048943 variant with generalized aggressive periodontitis (GAgP) and platelet activation and analyse whether its interaction with hyperlipidemia affects periodontal status in a Chinese population.
A case-control study of 224 GAgP patients and 139 healthy controls was conducted. The clinical parameters of probing depth (PD), attachment loss (AL) and bleeding index (BI) were recorded. Platelet count (PLT), platelet distribution width (PDW), platelet large cell ratio (PLCR), mean platelet volume (MPV), serum total cholesterol (TC), triacylglycerol (TG), high and low-density lipoprotein (HDL and LDL) were also measured. The CYP1A1 rs1048943 SNP was genotyped by time-of-flight mass spectrometry. Logistic and linear regression models were used to measure correlation.
The CYP1A1 rs1048943 AG/GG genotype was associated with GAgP (OR = 1.56, 95%CI: 1.01, 2.42), PD, AL and decreased PDW, PLCR and MPV after adjustment for covariates. Gene-lipid interactions were found between CYP1A1 rs1048943 and HDL for PD (P = 0.0033), BI (P = 0.0311) and AL (P = 0.0141) and between CYP1A1 rs1048943 and LDL for PD (P = 0.013) among patients with GAgP.
The G allele of the CYP1A1 rs1048943 gene was associated with GAgP, periodontal status and platelet-related inflammation status in a Chinese population. Hyperlipidemia could modulate the effect of CYP1A1 rs1048943 on the periodontal status of GAgP.
CYP1A1 rs1048943 多态性与牙周炎相关，但尚未研究其与侵袭性牙周炎（AgP）的关系。本研究旨在探讨 CYP1A1 基因 rs1048943 变异与广泛侵袭性牙周炎（GAgP）、血小板活化的关系，并分析其与高血脂的相互作用是否影响中国人群的牙周状况。
对 224 例 GAgP 患者和 139 例健康对照进行病例对照研究。记录探诊深度（PD）、附着丧失（AL）和出血指数（BI）等临床参数。还测量了血小板计数（PLT）、血小板分布宽度（PDW）、血小板大细胞比（PLCR）、平均血小板体积（MPV）、血清总胆固醇（TC）、三酰甘油（TG）、高低密度脂蛋白（HDL 和 LDL）。采用飞行时间质谱法对 CYP1A1 rs1048943 SNP 进行基因分型。采用逻辑回归和线性回归模型来测量相关性。
CYP1A1 rs1048943AG/GG 基因型与 GAgP 相关（OR=1.56，95%CI：1.01，2.42），经协变量调整后，与 PD、AL 和 PDW、PLCR、MPV 降低相关。在 GAgP 患者中，CYP1A1 rs1048943 与 HDL 之间存在基因-脂质相互作用，与 PD（P=0.0033）、BI（P=0.0311）和 AL（P=0.0141）有关，CYP1A1 rs1048943 与 LDL 之间存在基因-脂质相互作用，与 PD（P=0.013）有关。
CYP1A1 rs1048943 基因的 G 等位基因与中国人群的 GAgP、牙周状况和血小板相关炎症状态相关。高血脂可能调节 CYP1A1 rs1048943 对 GAgP 牙周状况的影响。
