啮齿动物的颅内药物治疗与疼痛测定
Intracranial Pharmacotherapy and Pain Assays in Rodents.
作者信息
Martinez Erik, Zhou Haocheng, Wang Jing
机构信息
Department of Anesthesiology, Perioperative Care and Pain Medicine, New York University School of Medicine.
Department of Anesthesiology, Perioperative Care and Pain Medicine, New York University School of Medicine; Department of Neuroscience and Physiology, New York University School of Medicine;
出版信息
J Vis Exp. 2019 Apr 9(146). doi: 10.3791/58473.
Pain is a salient sensory experience with affective and cognitive dimensions. However, central mechanisms for pain remain poorly understood, hindering the development of effective therapeutics. Intracranial pharmacology presents an important tool for understanding the molecular and cellular mechanisms of pain in the brain, as well as for novel treatments. Here we present a protocol that integrates intracranial pharmacology with pain behavior testing. Specifically, we show how to infuse analgesic drugs into a select brain region, which may be responsible for pain modulation. Furthermore, to determine the effect of the candidate drug in the central nerve system, pain assays are performed after intracranial treatment. Our results demonstrate that intracranial administration of analgesic drugs in a targeted region can provide relief of pain in rodents. Thus, our protocol successfully demonstrates that intracranial pharmacology, combined with pain behavior testing, can be a powerful tool for the study of pain mechanisms in the brain.
疼痛是一种具有情感和认知维度的显著感觉体验。然而，疼痛的中枢机制仍知之甚少，这阻碍了有效治疗方法的开发。颅内药理学是理解大脑中疼痛的分子和细胞机制以及新型治疗方法的重要工具。在此，我们介绍一种将颅内药理学与疼痛行为测试相结合的方案。具体而言，我们展示了如何将镇痛药注入可能负责疼痛调节的特定脑区。此外，为了确定候选药物在中枢神经系统中的作用，在颅内治疗后进行疼痛测定。我们的结果表明，在啮齿动物的靶向区域颅内给药镇痛药可以缓解疼痛。因此，我们的方案成功证明，颅内药理学与疼痛行为测试相结合，可以成为研究大脑疼痛机制的有力工具。
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