Kouker G, Jaeger K E
Appl Environ Microbiol. 1987 Jan;53(1):211-3. doi: 10.1128/aem.53.1.211-213.1987.
A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.
本文介绍了一种平板分析法，用于检测含有三油酰甘油和荧光染料罗丹明B的培养基中的细菌脂肪酶（EC 3.1.1.3）。底物水解会导致在紫外线照射下可见细菌菌落周围形成橙色荧光晕圈。来自无细胞培养上清液的脂肪酶活性对数与晕圈直径呈线性相关，从而能够对1至30纳卡特的脂肪酶活性进行定量。
