CSIR - Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar, Gujarat, 364 002, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201 002, India.
Biodegradation. 2024 Oct 29;36(1):4. doi: 10.1007/s10532-024-10101-5.
Polyvinyl chloride (PVC) is the third most produced synthetic plastic and releases the most harmful and lethal environmental component after incineration and landfilling. Few studies on microbial degradation of PVC have been reported but very little knowledge about the enzymes. In the present study, esterase enzyme was isolated and partially purified from marine bacterial isolates (T-1.3, BP-4.3 and S-237 identified as Vibrio sp., Alteromonas sp., and Cobetia sp., respectively) having the capability of PVC degradation. Initially, a plate assay was carried out for testing esterase production by studying bacteria using 1-naphthyl acetate as substrate. Enzyme assay showed higher production of esterase i.e. 0.57 U mL (2nd day), 0.46 U mL (2nd day) and 0.55 U mL (5th day) by bacterial isolate Vibrio sp., Alteromonas sp. and Cobetia sp., respectively incubated with PVC. Other enzymes like lipase, laccase and manganese peroxidase were much less or negligible compared to esterase enzyme production. Sephadex G-50 column purification had shown 58.62, 42.35 and 223.70 units mg of a specific activity by esterase for bacterial isolates Vibrio sp., Alteromonas sp. and Cobetia sp., respectively. Further, Sephadex G-50 column purification removed all the contamination and gave a clear appearance of the band at 38, 20 and 20 KD for bacterial isolates Vibrio sp., Alteromonas sp., and Cobetia sp., respectively. Esterase has shown maximum stability at a range of pH between 6.0 to 7.5, temperature between 30 to 35 °C and salinity concentration between 3 to 3.5 M for all bacterial isolates. In conclusion, esterase enzyme has promising potential to degrade PVC which can contribute to the decline the plastic pollution in an eco-friendly manner from the environment.
聚氯乙烯（PVC）是第三大生产合成塑料，在焚烧和填埋后释放出最有害和最致命的环境成分。虽然已经有一些关于聚氯乙烯微生物降解的研究，但对于相关酶的了解却非常有限。在本研究中，从具有 PVC 降解能力的海洋细菌分离物（分别鉴定为弧菌属、交替单胞菌属和科贝蒂亚菌属的 T-1.3、BP-4.3 和 S-237）中分离和部分纯化了酯酶。最初，通过使用 1-萘乙酸酯作为底物研究细菌，进行平板测定以测试酯酶的产生。酶测定表明，细菌分离物弧菌属、交替单胞菌属和科贝蒂亚菌属分别产生的酯酶产量较高，分别为 0.57 U mL（第 2 天）、0.46 U mL（第 2 天）和 0.55 U mL（第 5 天）。与酯酶酶生产相比，其他酶如脂肪酶、漆酶和锰过氧化物酶的产量要低得多或可以忽略不计。Sephadex G-50 柱纯化显示，细菌分离物弧菌属、交替单胞菌属和科贝蒂亚菌属的酯酶比活力分别为 58.62、42.35 和 223.70 单位 mg。此外，Sephadex G-50 柱纯化去除了所有污染物，并分别在 38、20 和 20 KD 处给出了细菌分离物弧菌属、交替单胞菌属和科贝蒂亚菌属的清晰条带。酯酶在所有细菌分离物的 pH 值范围为 6.0 至 7.5、温度范围为 30 至 35°C 和盐度浓度范围为 3 至 3.5 M 之间表现出最大稳定性。总之，酯酶具有降解 PVC 的巨大潜力，可以为以环保的方式减少环境中的塑料污染做出贡献。
