二酰基甘油诱导小鼠巨噬细胞中磷脂酰肌醇的脱酰作用和花生四烯酸的动员。与佛波酯诱导作用的比较。
Diacylglycerol induces deacylation of phosphatidylinositol and mobilization of arachidonic acid in mouse macrophages. Comparison with induction by phorbol diester.
作者信息
Emilsson A, Wijkander J, Sundler R
出版信息
Biochem J. 1986 Nov 1;239(3):685-90. doi: 10.1042/bj2390685.
Abstract
1,2-Dioctanoyl-sn-glycerol (2-50 microM) was found, like phorbol myristate acetate (greater than or equal to 3 nM) to stimulate phospholipase A-type cleavage of phosphatidylinositol and the release of arachidonic acid from macrophage phospholipids. The 1,3 isomer of dioctanoylglycerol was inactive, whereas racemic 1,2-dioctanoylglycerol was half as potent as the 1,2-sn enantiomer. Dioctanoylglycerol-induced deacylation of phosphatidylinositol was only partly dependent on extracellular calcium but was more severely inhibited by depletion of intracellular calcium. Chlorpromazine inhibited the deacylation of phosphatidylinositol, whereas inhibitors of cyclo-oxygenase and lipoxygenase were ineffective. Since both phorbol myristate acetate and 1,2-dioctanoyl-sn-glycerol are known to activate protein kinase C, the results suggest that this kinase is involved in the sequence of events leading to release of arachidonic acid in macrophages.
摘要
已发现1,2 - 二辛酰 - sn - 甘油（2 - 50微摩尔）与佛波醇肉豆蔻酸酯乙酸酯（大于或等于3纳摩尔）一样，能刺激磷脂酶A对磷脂酰肌醇的裂解，并从巨噬细胞磷脂中释放花生四烯酸。二辛酰甘油的1,3异构体无活性，而外消旋1,2 - 二辛酰甘油的效力仅为1,2 - sn对映体的一半。二辛酰甘油诱导的磷脂酰肌醇脱酰基作用仅部分依赖于细胞外钙，但更严重地受到细胞内钙耗竭的抑制。氯丙嗪抑制磷脂酰肌醇的脱酰基作用，而环氧化酶和脂氧合酶抑制剂则无效。由于已知佛波醇肉豆蔻酸酯乙酸酯和1,2 - 二辛酰 - sn - 甘油都能激活蛋白激酶C，结果表明该激酶参与了导致巨噬细胞中花生四烯酸释放的一系列事件。
相似文献
1
Diacylglycerol induces deacylation of phosphatidylinositol and mobilization of arachidonic acid in mouse macrophages. Comparison with induction by phorbol diester.二酰基甘油诱导小鼠巨噬细胞中磷脂酰肌醇的脱酰作用和花生四烯酸的动员。与佛波酯诱导作用的比较。
Biochem J. 1986 Nov 1;239(3):685-90. doi: 10.1042/bj2390685.
2
Evidence for a catalytic role of phospholipase A in phorbol diester- and zymosan-induced mobilization of arachidonic acid in mouse peritoneal macrophages.磷脂酶A在佛波酯和酵母聚糖诱导的小鼠腹腔巨噬细胞花生四烯酸动员中的催化作用证据。
Biochim Biophys Acta. 1986 May 21;876(3):533-42. doi: 10.1016/0005-2760(86)90041-x.
3
A phospholipase A2 hydrolyzing arachidonoyl-phospholipids in mouse peritoneal macrophages.一种在小鼠腹腔巨噬细胞中水解花生四烯酰磷脂的磷脂酶A2。
FEBS Lett. 1989 Feb 13;244(1):51-6. doi: 10.1016/0014-5793(89)81160-3.
4
A glycerol ether induces mobilization and 12-lipoxygenation of arachidonic acid in macrophages. Synergistic effect on mobilization and induction of leukotriene C formation by activators of protein kinase C.一种甘油醚可诱导巨噬细胞中花生四烯酸的动员和12-脂氧合作用。对蛋白激酶C激活剂介导的白三烯C形成的动员和诱导具有协同作用。
Agents Actions. 1990 Mar;29(3-4):247-53. doi: 10.1007/BF01966454.
5
Stimulation of phospholipid hydrolysis and arachidonic acid mobilization in human uterine decidua cells by phorbol ester.佛波酯对人子宫蜕膜细胞中磷脂水解和花生四烯酸动员的刺激作用。
Biochem J. 1987 Sep 15;246(3):705-13. doi: 10.1042/bj2460705.
6
A role for protein kinase C-mediated phosphorylation in the mobilization of arachidonic acid in mouse macrophages.蛋白激酶C介导的磷酸化在小鼠巨噬细胞花生四烯酸动员中的作用。
Biochim Biophys Acta. 1989 Jan 17;1010(1):78-87. doi: 10.1016/0167-4889(89)90187-0.
7
Differential effect of diacylglycerols and phorbol ester on arachidonic acid metabolism in bone marrow-derived macrophages.
Biol Chem Hoppe Seyler. 1990 Apr;371(4):369-74. doi: 10.1515/bchm3.1990.371.1.369.
8
Studies on the enzymatic pathways of calcium ionophore-induced phospholipid degradation and arachidonic acid mobilization in peritoneal macrophages.钙离子载体诱导腹膜巨噬细胞中磷脂降解和花生四烯酸动员的酶促途径研究。
Biochim Biophys Acta. 1985 Aug 30;846(2):265-74. doi: 10.1016/0167-4889(85)90074-6.
9
Diradylglycerols stimulate phospholipase A2 and subsequent exocytosis in ram spermatozoa. Evidence that the effect is not mediated via protein kinase C.二酰基甘油刺激公羊精子中的磷脂酶A2及随后的胞吐作用。有证据表明该效应不是通过蛋白激酶C介导的。
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj2970225.
10
Diacylglycerols mimic phorbol diester induction of leukemic cell differentiation.二酰基甘油模拟佛波酯诱导白血病细胞分化。
Proc Natl Acad Sci U S A. 1985 Feb;82(3):815-9. doi: 10.1073/pnas.82.3.815.
引用本文的文献
1
Requirement for diacylglycerol and protein kinase C in HeLa cell-substratum adhesion and their feedback amplification of arachidonic acid production for optimum cell spreading.HeLa细胞与基质黏附中对二酰基甘油和蛋白激酶C的需求及其对花生四烯酸产生的反馈放大作用以实现最佳细胞铺展。
Mol Biol Cell. 1993 Mar;4(3):271-81. doi: 10.1091/mbc.4.3.271.
2
Diradylglycerols stimulate phospholipase A2 and subsequent exocytosis in ram spermatozoa. Evidence that the effect is not mediated via protein kinase C.二酰基甘油刺激公羊精子中的磷脂酶A2及随后的胞吐作用。有证据表明该效应不是通过蛋白激酶C介导的。
Biochem J. 1994 Jan 1;297 ( Pt 1)(Pt 1):225-32. doi: 10.1042/bj2970225.
3
The diacylglycerols dioctanoylglycerol and oleoylacetylglycerol enhance prostaglandin synthesis by inhibition of the lysophosphatide acyltransferase.二酰基甘油二辛酰甘油和油酰乙酰甘油通过抑制溶血磷脂酰转移酶来增强前列腺素的合成。
Biochem J. 1987 Nov 1;247(3):773-7. doi: 10.1042/bj2470773.
4
Stimulation of phospholipid hydrolysis and arachidonic acid mobilization in human uterine decidua cells by phorbol ester.佛波酯对人子宫蜕膜细胞中磷脂水解和花生四烯酸动员的刺激作用。
Biochem J. 1987 Sep 15;246(3):705-13. doi: 10.1042/bj2460705.
5
Regulation of prostaglandin synthesis by protein kinase C in mouse peritoneal macrophages.蛋白激酶C对小鼠腹腔巨噬细胞中前列腺素合成的调节作用。
Biochem J. 1989 Jun 1;260(2):471-8. doi: 10.1042/bj2600471.
6
Regulation of eicosanoid synthesis in mouse peritoneal macrophages by protein kinase C.
Agents Actions. 1989 Jan;26(1-2):175-7. doi: 10.1007/BF02126599.
7
Regulation of intracellular free arachidonic acid in Aplysia nervous system.
J Membr Biol. 1990 Jul;116(3):249-60. doi: 10.1007/BF01868464.
8
A glycerol ether induces mobilization and 12-lipoxygenation of arachidonic acid in macrophages. Synergistic effect on mobilization and induction of leukotriene C formation by activators of protein kinase C.一种甘油醚可诱导巨噬细胞中花生四烯酸的动员和12-脂氧合作用。对蛋白激酶C激活剂介导的白三烯C形成的动员和诱导具有协同作用。
Agents Actions. 1990 Mar;29(3-4):247-53. doi: 10.1007/BF01966454.
本文引用的文献
1
THE DIFFERENTIATION OF MONONUCLEAR PHAGOCYTES. MORPHOLOGY, CYTOCHEMISTRY, AND BIOCHEMISTRY.单核吞噬细胞的分化。形态学、细胞化学与生物化学
J Exp Med. 1965 Jan 1;121(1):153-70. doi: 10.1084/jem.121.1.153.
2
3
Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.肿瘤促进剂佛波酯对钙激活的、磷脂依赖性蛋白激酶的直接激活作用。
J Biol Chem. 1982 Jul 10;257(13):7847-51.
4
1,2-Diacylglycerols do not potentiate the action of phospholipases A2 and C in human platelets.1,2 - 二酰基甘油不会增强磷脂酶A2和C在人血小板中的作用。
Biochem Biophys Res Commun. 1984 May 31;121(1):386-91. doi: 10.1016/0006-291x(84)90734-4.
5
6
The effects of lithium ion and other agents on the activity of myo-inositol-1-phosphatase from bovine brain.锂离子及其他试剂对牛脑肌醇-1-磷酸酶活性的影响。
J Biol Chem. 1980 Nov 25;255(22):10896-901.
7
The role of protein kinase C in cell surface signal transduction and tumour promotion.蛋白激酶C在细胞表面信号转导及肿瘤促进中的作用。
Nature. 1984;308(5961):693-8. doi: 10.1038/308693a0.
8
Inositol trisphosphate and diacylglycerol as second messengers.肌醇三磷酸和二酰甘油作为第二信使。
Biochem J. 1984 Jun 1;220(2):345-60. doi: 10.1042/bj2200345.
9
Thin-layer chromatography of the phosphoinositides.磷酸肌醇的薄层层析法。
J Lipid Res. 1968 Jul;9(4):532-3.
10
Phorbol esters and oleoyl acetoyl glycerol enhance release of arachidonic acid in platelets stimulated by Ca2+ ionophore A23187.佛波酯和油酰乙酰甘油可增强钙离子载体A23187刺激的血小板中花生四烯酸的释放。
J Biol Chem. 1985 Oct 15;260(23):12484-91.
Zotero 插件