Research Institute of Nyíregyháza, IAREF, University of Debrecen, P.O. Box 12, Nyíregyháza, 4400, Hungary.
Plant Mol Biol. 2019 Jul;100(4-5):511-525. doi: 10.1007/s11103-019-00876-0. Epub 2019 Apr 29.
In response to an ultrasound pulse, several hundred DEGs, including in response to stress, were up- or down-regulated in in vitro potato plantlets. Despite this abiotic stress, plantlets survived. Ultrasound (US) can influence plant growth and development. To better understand the genetic mechanism underlying the physiological response of potato to US, single-node segments of four-week-old in vitro plantlets were subjected to US at 35 kHz for 20 min. Following mRNA purification, 10 cDNA libraries were assessed by RNA-seq. Significantly differentially expressed genes (DEGs) were categorized by gene ontology or Kyoto Encyclopedia of Genes and Genomes identifiers. The expression intensity of 40,430 genes was studied. Several hundred DEGs associated with biosynthesis, carbohydrate metabolism and catabolism, cellular protein modification, and response to stress, and which were expressed mainly in the extracellular region, nucleus, and plasma membrane, were either up- or down-regulated in response to US. RT-qPCR was used to validate RNA-seq data of 10 highly up- or down-regulated DEGs, and both Spearman and Pearson correlations between SeqMonk LFC and RT-qPCR LFC were highly positive (0.97). This study examines how some processes evolved over time (0 h, 24 h, 48 h, 1 week and 4 weeks) after an abiotic stress (US) was imposed on in vitro potato explants, and provides clues to the temporal dynamics in DEG-based enzyme functions in response to this stress. Despite this abiotic stress, plantlets survived.
在体外马铃薯幼苗中，对超声脉冲的反应导致数百个 DEG（差异表达基因）上调或下调，包括对胁迫的反应。尽管存在这种非生物胁迫，但幼苗存活下来。超声（US）可以影响植物的生长和发育。为了更好地理解马铃薯对 US 生理反应的遗传机制，将 4 周龄体外幼苗的单节段用 35 kHz 的 US 处理 20 分钟。在 mRNA 纯化后，通过 RNA-seq 评估了 10 个 cDNA 文库。根据基因本体论或京都基因与基因组百科全书标识符对差异表达基因（DEG）进行分类。研究了 40430 个基因的表达强度。数百个与生物合成、碳水化合物代谢和分解代谢、细胞蛋白质修饰以及应激反应相关的 DEG，主要在细胞外区域、核和质膜中表达，其表达在 US 作用下上调或下调。使用 RT-qPCR 验证了 10 个高度上调或下调的 DEG 的 RNA-seq 数据，SeqMonk LFC 和 RT-qPCR LFC 之间的 Spearman 和 Pearson 相关性均为高度正相关（0.97）。本研究检查了在施加非生物胁迫（US）后，一些过程如何随着时间的推移（0 h、24 h、48 h、1 周和 4 周）而演变，为基于 DEG 的酶功能对这种胁迫的时间动态提供了线索。尽管存在这种非生物胁迫，但幼苗存活下来。
