College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou 730070, China.
College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Int J Biol Macromol. 2021 Jul 1;182:938-949. doi: 10.1016/j.ijbiomac.2021.04.094. Epub 2021 Apr 18.
The continuing increase in the global saline-alkali land area has made saline-alkali stress the principal abiotic stress limiting plant growth. Potato is the most important non-grain crop, and its production is also severely limited by saline-alkali stress. However, few studies have addressed the mechanism of saline-alkali tolerance of potato with a focus on its response to neutral salt NaCl stress, or its response to alkali stress. Recently, miRNA-mRNA analyses have helped advance our understanding of how plants respond to stress. Here, we have characterized the morphological, physiological, and transcriptome changes of tissue culture seedlings of potato variety "Qingshu No. 9" treated with NaHCO (for 0, 2, 6, and 24 h). We found that the leaves of tissue culture seedlings wilted and withered under alkali stress, and the contents of ABA, BRs, trehalose, and lignin in roots increased significantly. The contents of GAs decreased significantly. Subsequently, miRNA-seq analysis results identified 168 differentially expressed miRNAs (DEMIs) under alkali stress, including 21 exist miRNAs and 37 known miRNAs from 47 families and 110 novel miRNAs. The mRNA-seq results identified 5731 differentially expressed mRNAs (DEMs) under alkali stress. By miRNA-mRNA integrated analysis, were obtained 33 miRNA-target gene pairs composed of 20 DEMIs and 33 DEMs. Next, we identified the "phenylpropanoid biosynthesis", "plant hormone signal transduction", and "starch and sucrose metabolism" pathways as necessary for potato to respond to alkali stress. miR4243-x and novel-m064-5p were involved in the response of potato to alkali stress by their negative regulatory effects on shikimate O-hydroxycinnamoyltransferase (HCT) and sucrose-phosphate synthase (SPS) genes, respectively. The expression results of miRNA and mRNA were verified by quantitative real-time PCR (qRT-PCR). Our results clarify the mechanism of potato response to alkali stress at the miRNA level, providing new insights into the molecular mechanisms of potato's response to alkali stress. We report many candidate miRNAs and mRNAs for molecular-assisted screening and salt-alkali resistance breeding.
全球盐渍-碱土面积的持续增加，使盐渍-碱胁迫成为限制植物生长的主要非生物胁迫因素。马铃薯是最重要的非谷物作物，其生产也受到盐渍-碱胁迫的严重限制。然而，很少有研究关注马铃薯对中性盐 NaCl 胁迫的耐盐碱性机制，或对碱胁迫的响应机制。最近，miRNA-mRNA 分析有助于我们深入了解植物如何应对胁迫。在这里，我们对马铃薯品种“清水 9 号”的组织培养幼苗在 NaHCO3 处理（0、2、6 和 24 h）后的形态、生理和转录组变化进行了表征。我们发现，在碱胁迫下，组织培养幼苗的叶片萎蔫，根中 ABA、BRs、海藻糖和木质素的含量显著增加，GA 的含量显著降低。随后，miRNA-seq 分析结果鉴定出碱胁迫下有 168 个差异表达的 miRNAs（DEMIs），包括 21 个已知 miRNAs 和 37 个已知 miRNA 来自 47 个家族和 110 个新的 miRNAs。碱胁迫下的 mRNA-seq 结果鉴定出 5731 个差异表达的 mRNAs（DEMs）。通过 miRNA-mRNA 整合分析，获得了由 20 个 DEMIs 和 33 个 DEMs 组成的 33 个 miRNA-靶基因对。接下来，我们鉴定了“苯丙烷生物合成”、“植物激素信号转导”和“淀粉和蔗糖代谢”途径是马铃薯响应碱胁迫所必需的。miR4243-x 和 novel-m064-5p 通过对莽草酸 O-羟肉桂酰转移酶（HCT）和蔗糖-磷酸合酶（SPS）基因的负调控作用，分别参与了马铃薯对碱胁迫的响应。miRNA 和 mRNA 的表达结果通过定量实时 PCR（qRT-PCR）进行了验证。我们的研究结果在 miRNA 水平上阐明了马铃薯对碱胁迫的响应机制，为马铃薯对碱胁迫的分子机制提供了新的见解。我们报告了许多候选 miRNA 和 mRNAs，用于分子辅助筛选和耐盐-碱育种。
