1 Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, USA.
2 Department of Surgery, University of California, Irvine, USA.
Cell Transplant. 2019 Jul;28(7):967-972. doi: 10.1177/0963689719847460. Epub 2019 Apr 30.
The limited availability of human islets has led to the examination of porcine islets as a source of clinically suitable tissue for transplantation in patients with diabetes mellitus. Islets from porcine donors are commonly used in both and experiments studying diabetes mellitus. However, there are significant differences in quality and quantity of islet yield depending on donor pig age, as well as substantial differences in the costs of pancreas procurement in adult versus neonatal and juvenile pigs. In this study, we compared the total cost per islet of juvenile pig pancreata with that of neonatal and adult pigs. Although adult porcine pancreata yield, on average, more than five times the amount of islets than do juvenile and neonatal pancreata, we found that the high price of adult pigs led to the cost per islet being more than twice that of juvenile and neonatal islets (US $0.09 vs $0.04 and $0.02, respectively). In addition, neonatal and juvenile islets are advantageous in their scalability and retention of viability after culture. Our findings indicate that isolating neonatal and juvenile porcine islets is more cost-effective and scalable than isolating adult porcine islets.
胰岛的供应有限，促使人们研究猪胰岛作为糖尿病患者移植用的临床适用组织的来源。猪供体的胰岛通常用于糖尿病的 和 实验研究。然而，胰岛的产量在质量和数量方面都因供体猪的年龄而有显著差异，并且成年猪与新生猪和幼猪的胰腺获取成本也有很大差异。在这项研究中，我们比较了幼猪胰腺每个胰岛的总成本与新生猪和成年猪的成本。尽管成年猪的胰腺平均产生的胰岛数量是幼猪和新生猪胰腺的五倍以上，但我们发现，成年猪的高价格导致每个胰岛的成本超过幼猪和新生猪胰岛的两倍以上（分别为 0.09 美元、0.04 美元和 0.02 美元）。此外，新生猪和幼猪的胰岛在培养后具有更好的可扩展性和活力保持性。我们的研究结果表明，分离新生猪和幼猪的胰岛比分离成年猪的胰岛更具成本效益和可扩展性。
