Department of Food Science and Technology,University of West Attica,Greece.
Department of Wine, Vine and Beverage Sciences,University of West Attica,Greece.
J Dairy Res. 2019 May;86(2):238-241. doi: 10.1017/S0022029919000189. Epub 2019 Apr 30.
This Research Communication describes the adaptation and testing of an RP-HPLC method, previously tested for the determination of lactoferrin (LF) in whey, for its applicability to determine milk lactoferrin content. Milk samples of various species, namely, ovine, caprine, bovine, donkey and human milk, were tested. The advantage of this RP-HPLC method includes speed and convenience, as it does not include extensive pretreatment or separation steps. A simple pre-treatment step was added in order to remove fat and proteins of the casein family and the samples were tested. The results varied in terms of elution of the LF peak both between the milk of the different species as well as from the initial application on whey. The peak resolution was satisfactory in the cases of ovine, bovine and donkey milk samples while for caprine and human milk an interference with other peaks was observed. Nevertheless, quantification of LF was found possible for all samples. This new application of the modified method will allow the determination of LF in milk samples of the tested species either for everyday analysis or as a useful qualitative screening for presence or absence of LF.
本研究通讯描述了一种反相高效液相色谱 (RP-HPLC) 方法的适应性和测试，该方法之前已用于测定乳清中的乳铁蛋白 (LF)，现对其在测定牛奶中乳铁蛋白含量方面的适用性进行测试。对各种物种的牛奶样本，即绵羊、山羊、牛、驴和人乳进行了测试。该 RP-HPLC 方法的优点包括速度和便利性，因为它不包括广泛的预处理或分离步骤。添加了一个简单的预处理步骤，以去除乳清中的脂肪和酪蛋白家族的蛋白质，然后对样品进行测试。不同物种的牛奶以及最初在乳清上的应用之间，LF 峰的洗脱结果存在差异。在绵羊、牛和驴乳样本中，峰分辨率令人满意，而在山羊和人乳中，观察到与其他峰的干扰。然而，所有样本的 LF 定量都被认为是可能的。该改良方法的新应用将允许在测试物种的牛奶样本中测定 LF，无论是用于日常分析还是作为存在或不存在 LF 的有用定性筛选。
