Department of Neurology, The Second Affiliated Hospital of Qiqihar Medical University, Qiqihar, China.
J Cell Biochem. 2019 Sep;120(9):15538-15545. doi: 10.1002/jcb.28820. Epub 2019 Apr 30.
Glioma (GM) is an aggressive malignancy with high mortality rate across the world. Mounting studies demonstrates that abnormally expressed circular RNAs (circRNAs) act as important roles in tumor progression. In the current work, a novel circRNA, circ_0005198, was explored. Circ_0005198 level in GM tissue samples and cells was detected. The clinical implication of circ_0005198 was analyzed by Fisher's test and Kaplan-Meier analysis. In vitro experiments were carried out to elucidate the influence of circ_0005198 on GM cell proliferation, apoptosis, and metastatic properties. Luciferase reporter and rescue experiments were conducted to clear the mechanism of circ_0005198. The expression of circ_0005198 was enhanced in GM tumors and cells. Its expression in tumor specimens was related to clinical severity and poor prognosis. Functionally, circ_0005198 could remarkably boost cell growth, clone-forming ability, and metastatic properties and attenuate cell apoptosis in GM cells. What's more, circ_0005198 could directly sponge miR-1294 to exert oncogenic functions. In summary, this study might provide an effective therapeutic target for GM.
神经胶质瘤（GM）是一种具有全球高死亡率的侵袭性恶性肿瘤。越来越多的研究表明，异常表达的环状 RNA（circRNAs）在肿瘤进展中发挥着重要作用。在本研究中，我们研究了一种新型环状 RNA，circ_0005198。检测 GM 组织样本和细胞中的 circ_0005198 水平。通过 Fisher 检验和 Kaplan-Meier 分析分析 circ_0005198 的临床意义。进行体外实验阐明 circ_0005198 对 GM 细胞增殖、凋亡和转移特性的影响。进行荧光素酶报告和挽救实验以阐明 circ_0005198 的作用机制。circ_0005198 在 GM 肿瘤和细胞中表达增强。其在肿瘤标本中的表达与临床严重程度和预后不良相关。功能上，circ_0005198 可显著促进 GM 细胞的生长、克隆形成能力和转移特性，并抑制细胞凋亡。此外，circ_0005198 可以直接海绵吸附 miR-1294 发挥致癌功能。总之，本研究可能为 GM 提供一种有效的治疗靶点。
