a Vocational School, Medical Services and Techniques, Beykent University , Istanbul , Turkey.
b Faculty of Science, Chemistry Department , Erciyes University , Kayseri , Turkey.
J Biomater Sci Polym Ed. 2019 Aug;30(11):947-960. doi: 10.1080/09205063.2019.1612727. Epub 2019 May 21.
In this study, molecularly imprinted polymer membranes were synthesized for the recognition and adsorption of quercetin. For this, quercetin imprinted polymeric membranes [p(HEMA-MAH)] (Poly(2-hydroxyethyl methacrylate-co--methacryloly-l-histidinemethylester) were synthesized by UV polymerization technique using HEMA and MAH as monomers. Synthesized polymeric membranes were characterized with SEM, FTIR and swelling test. Characterized membranes were used for the direct adsorption of quercetin in a batch system. Quercetin adsorption conditions were optimized by using the quercetin imprinted polymeric membrane by altering the pH, temperature and initial quercetin concentration of the adsorption medium. Effect of adsorption time was also studied for up to 180 min. The optimum pH and temperature was determined between 4.0 and 45 °C. Maximum adsorbed amount of quercetin onto quercetin imprinted poly(HEMA-MAH) membrane was found to be as 299.6 mg/g membrane using the initial quercetin concentration of 2.0 mg/ml. Adsorbed quercetin was desorbed from the polymeric membranes with isopropyl alcohol with desorption yield of 98.3%. and repeated usability of the quercetin imprinted polymeric membranes was fallowed for 7 adsorption/desorption cycles. At the end of the 7 reuse, quercetin adsorption capacity of the quercetin imprinted poly(HEMA-MAH) membranes decreased only about 10%.
在这项研究中，合成了分子印迹聚合物膜以识别和吸附槲皮素。为此，通过 UV 聚合技术合成了以 HEMA 和 MAH 为单体的分子印迹聚合物膜[p(HEMA-MAH)]（聚（2-羟乙基甲基丙烯酸酯-co--甲基丙烯酸酰基-L-组氨酸甲酯）。合成的聚合物膜用 SEM、FTIR 和溶胀试验进行了表征。用所合成的印迹聚合物膜在批处理系统中直接吸附槲皮素，对吸附条件进行了优化，通过改变吸附介质的 pH、温度和初始槲皮素浓度。还研究了吸附时间长达 180 分钟的影响。最佳 pH 和温度确定在 4.0 和 45°C 之间。使用初始槲皮素浓度为 2.0mg/ml，发现槲皮素印迹聚（HEMA-MAH）膜对槲皮素的最大吸附量为 299.6mg/g 膜。用异丙醇从聚合物膜上解吸吸附的槲皮素，解吸率为 98.3%。对槲皮素印迹聚合物膜进行了 7 次吸附/解吸循环的重复使用性研究。在 7 次重复使用结束时，槲皮素印迹聚（HEMA-MAH）膜的槲皮素吸附容量仅下降了约 10%。
