Department of Biochemistry, Ege University Faculty of Science, Izmir, Turkey.
Turkish Health of Ministry, Turkish Medicines and Medical Devices Agency, Ankara, Turkey.
Environ Monit Assess. 2020 May 15;192(6):367. doi: 10.1007/s10661-020-08294-2.
We aimed to develop a molecularly imprinted polymeric systems with using penicillin G as a template molecule for removal of the antibiotic residues from environmental samples. Firstly, Pen-G-imprinted poly (2-hydroxyethyl methacrylate-N-methacryloyl-L-alanine) [p(HEMA-MAAL)] nanopolymers were synthesized by surfactant-free emulsion polymerization method. Then, template molecule (Pen-G) was extracted from nanopolymers. Synthesized nanopolymers were characterized by different methods such as Fourier-transform infrared spectroscopy (FTIR), elemental and zeta-size analysis, scanning electron microscope (SEM), and surface area calculations. Nanopolymers have 60.38 nm average size and 1034.22 m/g specific surface area. System parameters on Pen-G adsorption onto Pen-G imprint nanopolymers were investigated at different conditions. The specific adsorption value (Q) of molecularly impirinted p(HEMA-MAAL) nanopolymers was found 71.91 g/g for Pen-G in 5 mg/mL Pen-G initial concentration. Pen-G adsorption of molecularly imprinted nanopolymers was 15 times more than non-imprinted polymer. It is shown that obtained p(HEMA-MAAL) nanopolymer was a reuseable product which protected its adsorption capacity of 98.9% after 5th adsorption-desorption cycle. In conclusion, we suggest a method to develop a nanostructure, selective, low-cost molecularly imprinted polymeric systems with using penicillin G as a template molecule for removal of the antibiotic residues.
我们旨在开发一种分子印迹聚合物系统，以青霉素 G 作为模板分子，从环境样品中去除抗生素残留。首先，通过无表面活性剂乳液聚合方法合成了 Pen-G 印迹聚（2-羟乙基甲基丙烯酸酯-N-丙烯酰基-L-丙氨酸）[p(HEMA-MAAL)]纳米聚合物。然后，从纳米聚合物中提取模板分子（Pen-G）。通过不同的方法对合成的纳米聚合物进行了表征，例如傅里叶变换红外光谱（FTIR）、元素和zeta 尺寸分析、扫描电子显微镜（SEM）和表面积计算。纳米聚合物的平均粒径为 60.38nm，比表面积为 1034.22m/g。在不同条件下研究了 Pen-G 印迹纳米聚合物对 Pen-G 吸附的系统参数。在 5mg/mL 的初始 Pen-G 浓度下，Pen-G 印迹 p(HEMA-MAAL)纳米聚合物的特异性吸附值（Q）为 71.91g/g。印迹纳米聚合物对 Pen-G 的吸附量是非印迹聚合物的 15 倍。结果表明，所得到的 p(HEMA-MAAL)纳米聚合物是一种可重复使用的产品，在第 5 次吸附-解吸循环后，其吸附容量保持在 98.9%。总之，我们提出了一种以青霉素 G 为模板分子，开发具有选择性、低成本的纳米结构分子印迹聚合物系统的方法，用于去除抗生素残留。
