Burgos H
Eur J Clin Invest. 1986 Dec;16(6):486-93. doi: 10.1111/j.1365-2362.1986.tb02166.x.
Angiogenic and growth promoting factors from human amniochorion and placenta at term were released mostly as high molecular weight components (factor-carrier protein higher than 100,000 molecular weight) by extraction with 10% propan-2-ol, distilled water, and 50 mmol l-1 Tris/HCl pH 7.2 containing 50-150 mmol l-1 NaCl. They were isolated from the extracting media by adsorption on DEAE-Sepharose CL6B, a chromatographic agar based anion exchanger, and fractionated by chromatographic permeation on dextran gel Sephacryl S-300 yielding a low molecular weight component (between 400 and 1100 mol wt) with angiogenic and mitogenic capacities. Chromatographic behaviour and physio-chemical characteristics suggest it may be a peptide. Presence of an angiogenic and mitogenic factor in human amniochorion may explain the profuse neovascular formation and increased rate of healing obtained in the treatment of chronic ulcers by application of amniochorionic membranes as biological dressings. Preparation of purified angiogenic factor, on the other hand, opens the possibility of its wider application in the treatment of burns, open wounds and denuded areas in general.
足月时人羊膜绒毛膜和胎盘的血管生成及生长促进因子，通过用10%异丙醇、蒸馏水以及含50 - 150 mmol l-1氯化钠的50 mmol l-1 Tris/HCl pH 7.2提取，大多以高分子量成分（分子量高于100,000的因子 - 载体蛋白）形式释放。它们通过吸附在DEAE - Sepharose CL6B（一种基于琼脂糖的离子交换剂）上从提取介质中分离出来，并通过葡聚糖凝胶Sephacryl S - 300上的色谱渗透进行分级分离，得到一种具有血管生成和促有丝分裂能力的低分子量成分（分子量在400至1100之间）。色谱行为和物理化学特征表明它可能是一种肽。人羊膜绒毛膜中血管生成和促有丝分裂因子的存在，可能解释了通过应用羊膜绒毛膜作为生物敷料治疗慢性溃疡时大量新生血管形成以及愈合速度加快的现象。另一方面，纯化血管生成因子的制备为其在烧伤、开放性伤口和一般裸露区域治疗中的更广泛应用开辟了可能性。
