Lisniak I A
Eksp Onkol. 1987;9(4):72-3.
A fraction with the angiogenetic activity was obtained by the methods of the gel-chromatography on G-100 and the ion-exchange chromatography on DEAE-cellulose. Treatment of this fraction with the 10% water isopropanol solution has led to dissociation of the obtained material with a release of a low-molecular-weight angiogenic factor with a high angiogenic activity when tested on the chorioallantoic membrane. The molecular weight of this factor (300-600 daltons) was estimated by the chromatography on the biogel P2.
通过在G - 100上进行凝胶色谱法和在DEAE - 纤维素上进行离子交换色谱法获得了具有血管生成活性的级分。用10%水异丙醇溶液处理该级分导致所得物质解离，释放出一种低分子量血管生成因子，该因子在绒毛尿囊膜上测试时具有高血管生成活性。通过在生物凝胶P2上进行色谱法估计该因子的分子量（300 - 600道尔顿）。
