Kylla Hosterson, Dutta Tapan K, Roychoudhury Parimal, Subudhi Prasant K
Department of A.H and Veterinary, Disease Investigation Office, Meghalaya, Shillong, India.
Department of Veterinary Microbiology, Central Agricultural University, Aizawl, Mizoram, India.
Vet World. 2019;12(2):224-230. doi: 10.14202/vetworld.2019.224-230. Epub 2019 Feb 9.
This study aimed to study the prevalence of the coinfection of enteric bacterial and viral pathogens, namely , , and from fecal samples of pre-weaned piglets in Northeast region of India.
A total of 457 fresh fecal samples were collected from piglets under 9 weeks old during 2013-2015 from organized (n=225) and unorganized (n=232) farms of Manipur, Meghalaya, Mizoram, and Nagaland. Samples were collected from diarrheic (n =339) and non-diarrheic (n=118) piglets including local indigenous (n=130) and crossbreed (n=327) piglets in different seasons during the study period. The samples were processed for the isolation of and and detection of their putative virulence genes by polymerase chain reaction (PCR). Samples were also processed for the detection of and by RNA-polyacrylamide agarose gel electrophoresis and reverse transcriptase-PCR (RT-PCR).
A total of 11 (2.40%) samples were found positive for two or more coinfecting enteric bacterial and viral pathogens. All the 11 positive fecal samples were recovered from diarrheic piglets. Typhimurium (enterotoxin, gene) and genogroup 1 were found to be more frequent as coinfecting agents. Coinfection was recorded higher in unorganized (3.87%) compared to organized farm (0.88%). Again, higher detection was recorded in crossbreed (2.75%) than local indigenous piglets (1.53%). The occurrence of coinfection was found to be more common during summer (4.68%) followed by winter (2.27%) season.
The present study highlighted the significance of , , , and as important diarrheagenic pathogens causing coinfection in piglets in Northeast region of India. Probably, this is the first systematic study of the coinfection of four important diarrheagenic bacterial and viral agents associated with piglet diarrhea in India.
本研究旨在调查印度东北地区断奶前仔猪粪便样本中肠道细菌和病毒病原体（即 、 和 ）混合感染的发生率。
2013年至2015年期间，从曼尼普尔邦、梅加拉亚邦、米佐拉姆邦和那加兰邦的规模化养殖场（n = 225）和非规模化养殖场（n = 232）收集了457份9周龄以下仔猪的新鲜粪便样本。在研究期间的不同季节，从腹泻仔猪（n = 339）和非腹泻仔猪（n = 118）中采集样本，包括本地土种仔猪（n = 130）和杂交仔猪（n = 327）。对样本进行处理以分离 和 ，并通过聚合酶链反应（PCR）检测其假定的毒力基因。还通过RNA-聚丙烯酰胺琼脂糖凝胶电泳和逆转录聚合酶链反应（RT-PCR）对样本进行处理以检测 和 。
共发现11份（2.40%）样本存在两种或更多种肠道细菌和病毒病原体混合感染呈阳性。所有11份阳性粪便样本均来自腹泻仔猪。鼠伤寒沙门氏菌（肠毒素， 基因）和1型基因组被发现是更常见的混合感染病原体。非规模化养殖场（3.87%）的混合感染率高于规模化养殖场（0.88%）。此外，杂交仔猪（2.75%）的检测率高于本地土种仔猪（1.53%）。发现混合感染在夏季（4.68%）最为常见，其次是冬季（2.27%）。
本研究强调了 、 、 和 作为印度东北地区仔猪混合感染的重要致腹泻病原体的重要性。这可能是印度首次对与仔猪腹泻相关的四种重要致腹泻细菌和病毒病原体混合感染进行的系统研究。
