Zhang Dongyan, Liu Hui, Wang Sixin, Zhang Wei, Wang Jing, Tian Hongwu, Wang Yamin, Ji Haifeng
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
Beijing Research Center of Intelligent Equipment for Agriculture, Beijing, China.
Front Microbiol. 2019 Apr 16;10:785. doi: 10.3389/fmicb.2019.00785. eCollection 2019.
has a positive effect on the host intestinal microbiota. In piglets, dietary supplementation with affects general health and plays an important role in nutrient digestion and fermentation. However, this association requires further investigation. Here, we studied newborn piglets from 12 litters. The nursed piglets were given a creep feed beginning on day 10 and weaned at day 30. Piglets were fed either a control basic diet or a diet including supplementation with ZLR003 at 6.0 × 10 CFU/g feed. At day 30 and 60, feces samples were taken and used for sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. At day 60, feces samples and serum samples were also taken and used to measure the short chain fatty acids (SCFAs) and to detect long chain fatty acids (LCFAs) and free amino acids (FAAs), respectively. The results revealed that ZLR003 could improve piglet fecal microbiota composition, especially at the end of weaned period. The concentrations of lactic acid and butyric acid in feces were higher, and acetic acid concentration was lower in the ZLR003 group compared with the control group ( < 0.05). The serum polyunsaturated fatty acids C18:2n6c, C18:3n3, C20:4n6, and C22:6n3 were significantly higher ( < 0.05), as were the serum FAAs Gly, Ala, Val, Iso, Asn, Asp, Glu, Met, Phe, and Leu ( < 0.05), in the group compared with the control group. A correlation analysis revealed that the genera _UCG-010 and _UCG-014 had a negative correlation with the SCFAs content in feces, the genus _9 had a higher positive correlation with C18:2n6c, and the genera and had a more positive significant effect on the serum FAAs content in weaned piglets in the ZLR003 group compared with the control group. In conclusion, ZLR003 influenced the fecal microbiota composition of piglets, and its effects were related to the metabolism of SCFAs, LCFAs, and FAAs. Our findings will help facilitate the application of strains in pig production.
对宿主肠道微生物群有积极影响。在仔猪中，日粮添加……影响总体健康，并在营养物质消化和发酵中发挥重要作用。然而，这种关联需要进一步研究。在此，我们研究了12窝新生仔猪。哺乳仔猪从第10天开始给予开食料，并在第30天断奶。仔猪分别饲喂对照基础日粮或含6.0×10 CFU/g饲料的ZLR003补充日粮。在第30天和第60天采集粪便样本，用于16S rRNA基因V3-V4高变区测序。在第60天，还采集粪便样本和血清样本，分别用于测量短链脂肪酸（SCFAs）以及检测长链脂肪酸（LCFAs）和游离氨基酸（FAAs）。结果显示，ZLR003可改善仔猪粪便微生物群组成，尤其是在断奶期结束时。与对照组相比，ZLR003组粪便中乳酸和丁酸浓度较高，乙酸浓度较低（P<0.05）。与对照组相比，ZLR003组血清多不饱和脂肪酸C18:2n6c、C18:3n3、C20:4n6和C22:6n3显著更高（P<0.05），血清FAAs甘氨酸、丙氨酸、缬氨酸、异亮氨酸、天冬酰胺、天冬氨酸、谷氨酸、蛋氨酸、苯丙氨酸和亮氨酸也显著更高（P<0.05）。相关性分析显示，UCG-010属和UCG-014属与粪便中SCFAs含量呈负相关，9属与C18:2n6c呈较高正相关，与对照组相比，ZLR003组的……属和……属对断奶仔猪血清FAAs含量有更显著的正向影响。总之，ZLR003影响仔猪粪便微生物群组成，其作用与SCFAs、LCFAs和FAAs的代谢有关。我们的研究结果将有助于促进……菌株在养猪生产中的应用。
