Laboratory of Feed Biotechnology, State Key Lab. of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing, 100083, China.
Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China.
Sci Rep. 2017 Nov 17;7(1):15816. doi: 10.1038/s41598-017-16158-y.
The small intestine plays an essential role in the health and well-being of animals. Previous studies have shown that Lactobacillus has a protective effect on intestinal morphology, intestinal epithelium integrity and appropriate maturation of gut-associated tissues. Here, gene expression in jejunum tissue of weaned piglets was investigated by RNA-seq analysis after administration of sterile saline, Lactobacillus reuteri, or an antibiotic (chlortetracycline). In total, 401 and 293 genes were significantly regulated by chlortetracycline and L. reuteri, respectively, compared with control treatment. Notably, the HP, NOX1 and GPX2 genes were significantly up-regulated in the L. reuteri group compared with control, which is related to the antioxidant ability of this strain. In addition, the expression of genes related to arachidonic acid metabolism and linoleic acid metabolism enriched after treatment with L. reuteri. The fatty acid composition in the jejunum and colon was examined by GC-MS analysis and suggested that the MUFA C18:1n9c, and PUFAs C18:2n6c and C20:4n6 were increased in the L. reuteri group, verifying the GO enrichment and KEGG pathway analyses of the RNA-seq results. The results contribute to our understanding of the probiotic activity of this strain and its application in pig production.
小肠在动物的健康和福祉中起着至关重要的作用。先前的研究表明，乳酸菌对肠道形态、肠道上皮完整性和肠道相关组织的适当成熟具有保护作用。在这里，通过 RNA-seq 分析研究了无菌生理盐水、鼠李糖乳杆菌或抗生素（金霉素）给药后断奶仔猪空肠组织中的基因表达。与对照处理相比，金霉素和鼠李糖乳杆菌分别分别有 401 个和 293 个基因显著调节。值得注意的是，与对照相比，HP、NOX1 和 GPX2 基因在鼠李糖乳杆菌组中显著上调，这与该菌株的抗氧化能力有关。此外，用鼠李糖乳杆菌处理后，与花生四烯酸代谢和亚油酸代谢相关的基因表达富集。通过 GC-MS 分析检查空肠和结肠中的脂肪酸组成，并表明在鼠李糖乳杆菌组中 MUFA C18:1n9c、多不饱和脂肪酸 C18:2n6c 和 C20:4n6 增加，验证了 RNA-seq 结果的 GO 富集和 KEGG 途径分析。这些结果有助于我们了解该菌株的益生菌活性及其在猪生产中的应用。
