经鬼笔环肽处理的肌动蛋白丝在超声处理后的恢复过程中的重新退火及其受β-肌动蛋白抑制的情况。

Reannealing of phalloidin-treated actin filaments during recovery after sonication and its inhibition by beta-actinin.

作者信息

Oosawa M, Maruyama K

出版信息

FEBS Lett. 1987 Mar 23;213(2):433-7. doi: 10.1016/0014-5793(87)81537-5.

Abstract

Phalloidin (2 mol per mol actin)-treated pyrenyl F-actin showed a critical concentration of 1.8 microM in the presence of 10 mM KCl, 0.2 mM ADP, and 5 mM Tris-HCl buffer, pH 8.0 at 25 degrees C. The filament weight concentration did not change at all during and after sonication, yet degrees of flow birefringence increased and the filament number concentration decreased after the termination of sonication. The latter changes were not affected by EDTA, but inhibited by beta-actinin. These observations suggest that reannealing of short pieces of phalloidin-treated actin filaments fragmented during sonication takes place during recovery after sonication.

摘要

在含有10 mM氯化钾、0.2 mM二磷酸腺苷以及5 mM Tris - HCl缓冲液（pH 8.0）、25摄氏度的条件下，用鬼笔环肽（每摩尔肌动蛋白2摩尔）处理的芘基F - 肌动蛋白的临界浓度为1.8 microM。在超声处理期间及之后，丝状物重量浓度完全没有变化，然而在超声处理结束后，流动双折射程度增加，丝状物数量浓度降低。后一种变化不受乙二胺四乙酸（EDTA）影响，但受β - 辅肌动蛋白抑制。这些观察结果表明，在超声处理过程中碎片化的经鬼笔环肽处理的肌动蛋白丝短片段在超声处理后的恢复过程中发生了重新退火。