在黑腹果蝇中构建携带常染色体多巴脱羧酶基因的不稳定环状X染色体并分析多巴脱羧酶镶嵌体
Construction of an unstable Ring-X chromosome bearing the autosomal dopa decarboxylase gene in Drosophila melanogaster and analysis of Ddc mosaics.
作者信息
Gailey D A, Bordne D L, Vallés A M, Hall J C, White K
出版信息
Genetics. 1987 Feb;115(2):305-11. doi: 10.1093/genetics/115.2.305.
Abstract
An unstable Ring-X chromosome, Ddc+ -Ring-X carrying a cloned Dopa decarboxylase (Ddc) encoding segment was constructed. The construction involved a double recombination event between the unstable Ring-X, R(1)wvC and a Rod-X chromosome which contained a P-element mediated Ddc+ insert. The resulting Ddc+ -Ring-X chromosome behaves similarly to the parent chromosome with respect to somatic instability. The Ddc+ -Ring-X chromosome was used to generate Ddc mosaics. Analyses of Ddc mosaics revealed that while there was no absolute requirement for the Ddc+ expression in either the epidermis or the nervous system, very large mutant clones did affect the viability of the mosaic.
摘要
构建了一个不稳定的环形X染色体，即携带克隆的多巴脱羧酶（Ddc）编码片段的Ddc⁺ -环形X染色体。构建过程涉及不稳定的环形X染色体R(1)wvC与一个棒状X染色体之间的双重组事件，该棒状X染色体包含一个P因子介导的Ddc⁺插入片段。所得的Ddc⁺ -环形X染色体在体细胞不稳定性方面与亲本染色体表现相似。利用Ddc⁺ -环形X染色体产生了Ddc镶嵌体。对Ddc镶嵌体的分析表明，虽然在表皮或神经系统中对Ddc⁺表达没有绝对要求，但非常大的突变克隆确实会影响镶嵌体的活力。
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