Ishikawa T, Sakabe T, Nishiyama Y, Abe M, Takeshita H, Niwa M, Ozaki M
Nihon Yakurigaku Zasshi. 1986 Dec;88(6):425-31. doi: 10.1254/fpj.88.425.
The effects of S-Adenosyl-L-methionine (SAMe) on cerebral monoamine turnover at 60 min of reoxygenation after hypoxia (PaO2, 31-35 mmHg) for 15 min were studied in 44 rats anesthetized with nitrous oxide. The accumulations of monoamine metabolites: 3-methoxy 4-hydroxyphenylglycol (MHPG), 3,4-dihydroxyphenylacetic acid (DOPAC), homovanillic acid (HVA) and 5-hydroxyindoleacetic acid (5-HIAA) were determined after probenecid. The accumulations of noradrenaline (NA) and dopamine (DA) were also determined after pargyline. In the non-treated group, there was an impairment of degradation of NA to MHPG and of DA to DOPAC or HVA in the control group (no hypoxia). These changes were accompanied by higher levels of NA and DA in the cerebral cortex, hypothalamus and striatum than those of the control group. In the group treated with 100 mg/kg SAMe plus 38 mg/kg mannitol 3 min after the start of reoxygenation, there were no significant changes in these metabolites and amines. Mannitol alone did not cause significant changes. There were no changes in serotonin and 5-HIAA in any of the groups studied. The effects of SAMe were studied in an additional 16 awake rats. MHPG in all regions measured after probenecid in the rats treated with SAMe was higher than that without SAMe. It appears that SAMe ameliorates perturbation of cerebral catecholamine turnover produced by reoxygenation after hypoxia.
在44只接受一氧化二氮麻醉的大鼠中，研究了S-腺苷-L-蛋氨酸（SAMe）对缺氧（动脉血氧分压31 - 35 mmHg）15分钟后再给氧60分钟时脑单胺周转率的影响。用丙磺舒处理后，测定单胺代谢产物3-甲氧基-4-羟基苯乙二醇（MHPG）、3,4-二羟基苯乙酸（DOPAC）、高香草酸（HVA）和5-羟基吲哚乙酸（5-HIAA）的蓄积量。用优降宁处理后，还测定去甲肾上腺素（NA）和多巴胺（DA）的蓄积量。在未处理组中，对照组（无缺氧）存在NA降解为MHPG以及DA降解为DOPAC或HVA的障碍。这些变化伴随着大脑皮层、下丘脑和纹状体中NA和DA水平高于对照组。在再给氧开始3分钟后用100 mg/kg SAMe加38 mg/kg甘露醇处理的组中，这些代谢产物和胺类没有显著变化。单独使用甘露醇未引起显著变化。在所研究的任何组中，血清素和5-HIAA均无变化。在另外16只清醒大鼠中研究了SAMe的作用。用SAMe处理的大鼠经丙磺舒处理后，所有测量区域的MHPG均高于未用SAMe处理的大鼠。似乎SAMe改善了缺氧后再给氧引起的脑儿茶酚胺周转率紊乱。
