a Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory , College of Plant Science and Technology, Huazhong Agricultural University , Wuhan , P R China.
Chronobiol Int. 2019 Jul;36(7):969-978. doi: 10.1080/07420528.2019.1603158. Epub 2019 May 1.
The rice stem borer, Walker, is one of the most important global agricultural pests. has distinct rice and water-oat host populations. Asynchrony in sexual activity is thought to be the main factor maintaining reproductive segregation between these populations, particularly the obvious difference in the circadian rhythm of female calling activity between populations. However, the mechanism responsible for this difference in the timing of female calling is poorly understood. The circadian clock is an essential regulator of daily behavioral rhythms in insects, including female calling. We investigated the variation in circadian clock genes of the rice and water-oat populations of . We did this by comparing deduced amino acid sequences and the expression patterns of seven circadian clock genes (, and ) between females from each population. We found that the two populations had different variants of the and genes and differed in the expression of and . This suggests that population-related variation in the circadian clock genes and could be responsible for the different circadian rhythms of female calling in these host population of . These results provide new insights into the molecular mechanisms underlying asynchronous sexual activity in insect populations and suggest new topics for future research on the origins and maintenance of population differentiation in insects.
稻纵卷叶螟，沃克，是最重要的全球性农业害虫之一。 有明显的水稻和水燕麦寄主种群。性活动的不同步被认为是维持这些种群生殖隔离的主要因素，特别是种群间雌虫鸣叫活动的昼夜节律明显不同。然而，导致这种雌虫鸣叫时间差异的机制尚不清楚。生物钟是昆虫包括雌虫鸣叫在内的日常行为节律的重要调节者。我们研究了 的水稻和水燕麦种群的生物钟基因的变化。我们通过比较每个种群中来自雌虫的七个生物钟基因（, 和 ）的推导氨基酸序列和表达模式来做到这一点。我们发现，这两个种群的 和 基因有不同的变体， 和 的表达也不同。这表明生物钟基因 和 的种群相关变异可能是这些宿主种群中雌虫鸣叫昼夜节律不同的原因。这些结果为昆虫种群中异步性活动的分子机制提供了新的见解，并为昆虫种群分化的起源和维持的未来研究提出了新的课题。
