Strigunkova T F, Lavrentiev G A, Otroshchenko V A
J Mol Evol. 1986;23(4):290-3. doi: 10.1007/BF02100636.
The present work deals with the processes involved in the abiogenic polycondensation of nucleotides adsorbed on the clay mineral kaolinite under the action of ultraviolet (UV) radiation. The dependence of the yield of synthesis products on irradiation dose was studied. The maximum yield corresponds to a 6-h exposure. The newly synthesized substances were analyzed by ion-exchange chromatography. Some fractions were studied for the type of bonds they contained by venom phosphodiesterase and RNase T2 enzymatic hydrolysis. It was determined that some of the products synthesized by exposure of AMP adsorbed on the surface of clay particles to UV radiation may be looked upon as oligonucleotides in which some fragments have 2'-5'-bonded and others 3'-5'-bonded nucleotides.
本研究涉及在紫外线（UV）辐射作用下，吸附在粘土矿物高岭石上的核苷酸发生非生物多聚缩合的过程。研究了合成产物产率对辐照剂量的依赖性。最大产率对应6小时的照射时间。通过离子交换色谱法对新合成的物质进行了分析。通过蛇毒磷酸二酯酶和RNase T2酶促水解，研究了部分馏分所含的键型。结果表明，吸附在粘土颗粒表面的AMP经紫外线照射合成的一些产物可被视为寡核苷酸，其中一些片段具有2'-5'-键合的核苷酸，另一些片段具有3'-5'-键合的核苷酸。
