Loukas M, Delidakis C, Kafatos F C
J Mol Evol. 1986;24(1-2):174-88. doi: 10.1007/BF02099965.
Comparative, quantitative Southern analysis of genomic DNA, using single-copy sequence probes, potentially is valuable for phylogenetic analysis. We have examined 27 Drosophila species, belonging to two subgenera, seven species groups, and ten subgroups, using a variety of cloned and characterized probes: twelve cloned sequences from D. melanogaster, two from D. pseudoobscura, and two from D. grimshawi. The data are generally congruent with accepted phylogenetic relationships in Drosophila, and confirm or clarify some previously uncertain relationships. The potential and limitations of the method are discussed.
使用单拷贝序列探针，对基因组DNA进行比较性的定量Southern分析，在系统发育分析中可能具有重要价值。我们使用了多种已克隆并表征的探针，检测了27种果蝇，这些果蝇分属于两个亚属、七个物种组和十个亚组：其中有来自黑腹果蝇的12个克隆序列、来自拟暗果蝇的2个克隆序列以及来自格氏果蝇的2个克隆序列。这些数据总体上与果蝇公认的系统发育关系相符，并证实或澄清了一些之前不确定的关系。本文还讨论了该方法的潜力和局限性。
