Faculty of Bioscience Engineering, Department of Crop Protection Chemistry, Ghent University, Ghent, Belgium.
Institute for Interdisciplinary Research and Consultancy Services, University for Development Studies, Tamale, Ghana.
Food Addit Contam Part B Surveill. 2019 Dec;12(4):231-235. doi: 10.1080/19393210.2019.1609098. Epub 2019 May 2.
Glyphosate is used in Ghana on many crops including yam. There is the suspicion that there could be residue problems in the yam crop. Glyphosate as a polar compound is noted for its difficulty to be analysed in biological matrices. In this study, the method for glyphosate analysis based on FMOC-Cl derivatization was modified with a clean-up (CHCl) step and validated to analyse glyphosate in yam by LC-MS/MS. The results showed that the validated method was efficient for the analysis of glyphosate in yam, with recoveries of 34%, linearity of 0.997, RSD of 7%, LOD of 0.04 mg kg, and LOQ of 0.12 mg kg. Out of 68 samples analysed from a field experiment and from markets, glyphosate was detected in 14 samples, but at levels below the LOQ. It is concluded that the yam contained glyphosate residues at very low levels which may not pose threat to human health.
在加纳，草甘膦被广泛用于多种作物，包括山药。人们怀疑山药作物中可能存在残留问题。草甘膦作为一种极性化合物，其在生物基质中的分析难度较大。本研究对基于 FMOC-Cl 衍生化的草甘膦分析方法进行了修改，并增加了净化（CHCl）步骤，通过 LC-MS/MS 对山药中的草甘膦进行了验证分析。结果表明，该验证方法对山药中草甘膦的分析非常有效，回收率为 34%，线性度为 0.997，RSD 为 7%，LOD 为 0.04mg/kg，LOQ 为 0.12mg/kg。在田间试验和市场上分析的 68 个样本中，有 14 个样本检测到草甘膦，但含量均低于 LOQ。因此可以得出结论，山药中草甘膦残留水平非常低，可能不会对人体健康构成威胁。
