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[添加过氧化氢酶至罗-琴培养基对结核分枝杆菌生长速率的影响]

[The effect of catalase added to Löwenstein-Jensen medium on the rate of growth of M. tuberculosis].

作者信息

Durmaz R, Gürel M, Gökoğlu M, Durmaz B

出版信息

Mikrobiyol Bul. 1986 Jul;20(3):160-4.

PMID:3104733
Abstract

In this study, the effect of catalase on the rate of growth Mycobacterium tuberculosis has been investigated. Thirty-five sputa were inoculated on the Löwenstein-Jensen medium and Löwenstein-Jensen medium modified by the addition of catalase 4 mcg/ml. Only four sputa were observed to have an early growth on the Löwenstein-Jensen medium with catalase five days than Löwenstein-Jensen medium. Of the 55 strains grown in the Löwenstein-Jensen medium the following results were obtained from the inoculations made in the Löwenstein-Jensen with catalase and Löwenstein-Jensen medium. At the end of a three week incubation in a medium with 5-10% CO2, a growth of 32 strains (58%) in the Löwenstein-Jensen medium with catalase and a growth of 21 strains (38%) in the Löwenstein-Jensen medium were observed Eleven strains (20%) were observed in the Löwenstein-Jensen medium with catalase and B strains (14%) in the Löwenstein-Jensen medium during the same time in normal atmosphere.

摘要

在本研究中,已对过氧化氢酶对结核分枝杆菌生长速率的影响进行了调查。将35份痰液接种在洛温斯坦-詹森培养基和添加了4微克/毫升过氧化氢酶的改良洛温斯坦-詹森培养基上。仅观察到4份痰液在添加过氧化氢酶的洛温斯坦-詹森培养基上比在洛温斯坦-詹森培养基上提前5天生长。在洛温斯坦-詹森培养基中培养的55株菌株中,从接种于添加过氧化氢酶的洛温斯坦-詹森培养基和洛温斯坦-詹森培养基上获得了以下结果。在含有5-10%二氧化碳的培养基中培养三周后,观察到添加过氧化氢酶的洛温斯坦-詹森培养基中有32株菌株生长(58%),洛温斯坦-詹森培养基中有21株菌株生长(38%)。在正常大气环境下的同一时间,在添加过氧化氢酶的洛温斯坦-詹森培养基中观察到11株菌株(20%),在洛温斯坦-詹森培养基中观察到8株菌株(14%)。 (注:原文中“B strains”可能有误,推测为“8 strains”,已按此翻译)

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