Department of Anatomy, Histology and Embryology, University of Split, School of Medicine, Split, Croatia; University of Mostar, School of Medicine, Mostar, Bosnia and Herzegovina.
University of Mostar, School of Medicine, Mostar, Bosnia and Herzegovina; University Hospital Center Mostar, Mostar, Bosnia and Herzegovina.
Acta Histochem. 2019 Jul;121(5):531-538. doi: 10.1016/j.acthis.2019.04.011. Epub 2019 Apr 29.
Present study analyses the co-localisation of RIP5 with FGFR1, FGFR2 and HIP2 in the developing kidney, as RIP5 is a major determinant of urinary tract development, downstream of FGF-signaling.
Paraffin embedded human kidney tissues of 16 conceptuses between the 6th-22th developmental week were analysed using double-immunofluorescence method with RIP5/FGFR1/FGFR2 and HIP2 markers. Quantification of positive cells were performed using Kruskal-Wallis test.
In the 6th week of kidney development RIP5 (89.6%) and HIP2 (39.6%) are strongly expressed in the metanephric mesenchyme. FGFR1 shows moderate/strong expression in the developing nephrons (87.3%) and collecting ducts (70.5%) (p < 0.05). RIP5/FGFR1 co-localized at the marginal zone and the ureteric bud with predominant FGFR1 expression. FGFR2 (26.1%) shows similar expression pattern as FGFR1 (70.5%) in the same kidney structures. RIP5/FGFR2 co-localized at the marginal zone and the collecting ducts (predominant expression of FGFR2). HIP2 is strongly expressed in collecting ducts (96.7%), and co-localized with RIP5. In 10th week, RIP5 expression decrease (74.2%), while the pattern of expression of RIP5 and FGFR1 in collecting ducts (33.4% and 91.9%) and developing nephrons (21.9% and 32.4%) (p < 0.05) is similar to that in the 6th developmental week. Ureter is moderately expressing RIP5 while FGFR1 is strongly expressed in the ureteric wall. FGFR2 is strongly expressed in the collecting ducts (84.3%) and ureter. HIP2 have 81.1% positive cells in the collecting duct. RIP5/FGFR1 co-localize in collecting ducts and Henley's loop.
The expression pattern of RIP5, FGFR1, FGFR2 and HIP2 in the human kidney development might indicate their important roles in metanephric development and ureteric muscle layer differentiation through FGF signaling pathways.
本研究分析了 RIP5 与 FGFR1、FGFR2 和 HIP2 在发育肾脏中的共定位，因为 RIP5 是 FGF 信号下游尿路上皮发育的主要决定因素。
使用 RIP5/FGFR1/FGFR2 和 HIP2 标志物对 16 个人胚第 6-22 发育周的石蜡包埋人肾组织进行双免疫荧光分析。使用 Kruskal-Wallis 检验对阳性细胞进行定量。
在肾脏发育的第 6 周，RIP5（89.6%）和 HIP2（39.6%）在中肾间质中强烈表达。FGFR1 在发育中的肾单位（87.3%）和收集管（70.5%）中表达中度/强（p<0.05）。RIP5/FGFR1 共定位于边缘区和输尿管芽，FGFR1 表达为主。FGFR2（26.1%）在相同的肾脏结构中表现出与 FGFR1（70.5%）相似的表达模式。RIP5/FGFR2 共定位于边缘区和收集管（FGFR2 表达为主）。HIP2 在收集管中强烈表达（96.7%），并与 RIP5 共定位。在第 10 周，RIP5 表达下降（74.2%），但 RIP5 和 FGFR1 在收集管（33.4%和 91.9%）和发育肾单位（21.9%和 32.4%）中的表达模式相似（p<0.05）。输尿管中度表达 RIP5，而 FGFR1 在输尿管壁中强烈表达。FGFR2 在收集管（84.3%）和输尿管中强烈表达。HIP2 在收集管中有 81.1%的阳性细胞。RIP5/FGFR1 在收集管和 Henley 环中共定位。
RIP5、FGFR1、FGFR2 和 HIP2 在人肾发育中的表达模式表明它们在通过 FGF 信号通路的中肾发育和输尿管肌层分化中具有重要作用。
