National Centre for Biological Sciences (NCBS), TIFR, GKVK Campus, Bangalore 560065, Karnataka, India.
National Centre for Biological Sciences (NCBS), TIFR, GKVK Campus, Bangalore 560065, Karnataka, India; The University of Trans-Disciplinary Health Sciences & Technology (TDU), Yelahanka, Bangalore 560064, Karnataka, India.
Genomics. 2020 Jan;112(1):621-628. doi: 10.1016/j.ygeno.2019.04.014. Epub 2019 Apr 29.
Moringa oleifera is a plant well-known for its nutrition value, drought resistance and medicinal properties. cDNA libraries from five different tissues (leaf, root, stem, seed and flower) of M. oleifera cultivar Bhagya were generated and sequenced. We developed a bioinformatics pipeline to assemble transcriptome, along with the previously published M. oleifera genome, to predict 17,148 gene models. Few candidate genes related to biosynthesis of secondary metabolites, vitamins and ion transporters were identified. Expressions were further confirmed by real-time quantitative PCR experiments for few promising leads. Quantitative estimation of metabolites, as well as elemental analysis, was also carried out to support our observations. Enzymes in the biosynthesis of vitamins and metabolites like quercetin and kaempferol are highly expressed in leaves, flowers and seeds. The expression of iron transporters and calcium storage proteins were observed in root and leaves. In general, leaves retain the highest amount of small molecules of interest.
辣木（Moringa oleifera）是一种以其营养价值、耐旱性和药用特性而闻名的植物。从辣木（Moringa oleifera） Bhagya 品种的五个不同组织（叶、根、茎、种子和花）中生成和测序 cDNA 文库。我们开发了一个生物信息学管道来组装转录组，以及之前发表的辣木（Moringa oleifera）基因组，以预测 17,148 个基因模型。鉴定了一些与次生代谢物、维生素和离子转运体生物合成相关的候选基因。通过实时定量 PCR 实验进一步证实了少数有前途的线索。还进行了代谢物的定量估计以及元素分析，以支持我们的观察。在叶子、花朵和种子中，维生素和代谢物如槲皮素和山奈酚生物合成的酶表达水平较高。在根和叶子中观察到铁转运蛋白和钙储存蛋白的表达。总的来说，叶子保留了最多的感兴趣的小分子。
