用于[具体内容]的联合转录组组装和功能注释的数据集。 （你原文中“and”前面缺少具体信息）
Dataset for the combined transcriptome assembly of and functional annotation.
作者信息
Shafi K Mohamed, Joshi Adwait G, Meenakshi Iyer, Pasha Shaik Naseer, Harini K, Mahita Jarjapu, Sajeevan Radha Sivarajan, Karpe Snehal D, Ghosh Pritha, Nitish Sathyanarayanan, Gandhimathi A, Mathew Oommen K, Prasanna Subramanian Hari, Malini Manoharan, Mutt Eshita, Naika Mahantesha, Ravooru Nithin, Rao Rajas M, Shingate Prashant N, Sukhwal Anshul, Sunitha Margaret S, Upadhyay Atul K, Vinekar Rithvik S, Sowdhamini Ramanathan
机构信息
National Centre for Biological Sciences (TIFR), GKVK Campus, Bangalore 560065, Karnataka, India.
The University of Trans-Disciplinary Health Sciences & Technology (TDU), Yelahanka, Bangalore 560064, Karnataka, India.
出版信息
Data Brief. 2020 Mar 20;30:105416. doi: 10.1016/j.dib.2020.105416. eCollection 2020 Jun.
In this paper, we present the data acquired during transcriptome analysis of the Moringa oleifera [1] from five different tissues (root, stem, leaf, flower and seed) by RNA sequencing. A total of 271 million reads were assembled with an N50 of 2094 bp. The combined transcriptome was assessed for transcript abundance across five tissues. The protein coding genes identified from the transcripts were annotated and used for orthology analysis. Further, enzymes involved in the biosynthesis of select medicinally important secondary metabolites, vitamins and ion transporters were identified and their expression levels across tissues were examined. The data generated by RNA sequencing has been deposited to NCBI public repository under the accession number PRJNA394193 (https://www.ncbi.nlm.nih.gov/bioproject/PRJNA394193).
在本文中，我们展示了通过RNA测序对辣木（Moringa oleifera）[1]五个不同组织（根、茎、叶、花和种子）进行转录组分析期间获得的数据。总共组装了2.71亿条读数，N50为2094 bp。评估了五个组织中组合转录组的转录本丰度。对从转录本中鉴定出的蛋白质编码基因进行注释，并用于直系同源分析。此外，鉴定了参与选定的具有重要药用价值的次生代谢物、维生素生物合成的酶以及离子转运蛋白，并检测了它们在各组织中的表达水平。RNA测序产生的数据已存入NCBI公共数据库，登录号为PRJNA394193（https://www.ncbi.nlm.nih.gov/bioproject/PRJNA394193）。
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