School of Environmental and Chemical Engineering, Shanghai University, Shanghai, 200444, PR China; Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
Division of Molecular Surface Physics & Nanoscience, Department of Physics, Chemistry and Biology, Linköping University, Linköping 58183, Sweden.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Aug 5;219:232-239. doi: 10.1016/j.saa.2019.04.024. Epub 2019 Apr 15.
Hypochlorous acid (HClO) is one of the most important ROS (reactive oxygen species) and common pollutant in tap-water. However, the determination of HClO with fast response and high sensitivity/selectivity is still an urgent demanding. Here we fabricated a ratiometric fluorescent probe RC based on TBET (through-bond energy transfer) on the platform of coumarin and rhodamine with the thiosemicarbazide group as the linker. This probe could display the characteristic fluorescence emission of coumarin. Upon addition of HClO, the linker was reacted into an oxadiazole, resulting in the opening of spiro-ring of rhodamine. The resultant then gives ratiometric fluorogenic changes. The probe exhibits fast response and high selectivity and sensitivity towards HClO with a low limit of detection (~140 nM). Eventually, RC is successfully applicated for determining spiked HClO in water samples and imaging endogenous HClO in living cells.
次氯酸（HClO）是最重要的活性氧（ROS）和自来水中的常见污染物之一。然而，具有快速响应和高灵敏度/选择性的 HClO 的测定仍然是一个迫切的需求。在这里，我们基于香豆素和罗丹明构建了一种比率荧光探针 RC，平台上带有噻唑烷酮基团作为连接体的 TBET（键间能量转移）。该探针可以显示香豆素的特征荧光发射。加入 HClO 后，连接体反应成恶二唑，导致罗丹明的螺环打开。然后产生比率荧光变化。该探针对 HClO 具有快速响应和高选择性和灵敏度，检测限低（约 140 nM）。最终，RC 成功应用于测定水样中添加的 HClO 和在活细胞中成像内源性 HClO。
