College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul, 05029, Republic of Korea.
Sci Rep. 2019 May 2;9(1):6838. doi: 10.1038/s41598-019-43280-w.
The transferred microbiota from mother to baby constitutes the initial infant gastrointestinal microbiota and has an important influence on the development and health of infants in human. However, the reproductive tract microbiota of avian species and its inheritance have rarely been studied. We aimed to characterize the microbial community in the chicken reproductive tract and determine the origin of the chicken embryo gut microbiota. Microbiota in four different portions of chicken oviduct were determined using 16S rRNA metagenomic approach with the IonTorrent platform. Additionally, we analyzed the mother hen's magnum and cloaca, descendent egg, and embryo gut microbiota. The microbial composition and relative abundance of bacterial genera were stable throughout the entire chicken reproductive tract, without significant differences between the different parts of the oviduct. The chicken reproductive tract showed a relatively high abundance of Lactobacillus species. The number of bacterial species in the chicken reproductive tract significantly increased following sexual maturation. Core genera analysis detected 21 of common genera in the maternal magnum and cloaca, descendent egg shell, egg white, and embryo gut. Some elements of the maternal oviduct microbiota appear to be transferred to the embryo through the egg white and constitute most of the embryo gut bacterial population.
从母亲转移到婴儿的微生物群构成了婴儿最初的胃肠道微生物群，对人类婴儿的发育和健康有重要影响。然而，禽类生殖道微生物群及其遗传很少被研究。我们旨在描述鸡生殖道中的微生物群落，并确定鸡胚胎肠道微生物群的来源。我们使用 IonTorrent 平台的 16S rRNA 宏基因组方法来确定鸡输卵管的四个不同部位的微生物群。此外，我们分析了母鸡的喇叭和泄殖腔、后代鸡蛋和胚胎肠道微生物群。在整个鸡生殖道中，细菌属的微生物组成和相对丰度都很稳定，输卵管的不同部位之间没有显著差异。鸡生殖道中乳酸杆菌属的丰度相对较高。鸡生殖道中的细菌种类数量在性成熟后显著增加。核心属分析检测到 21 个常见属存在于母体喇叭和泄殖腔、后代蛋壳、蛋清和胚胎肠道中。一些母体输卵管微生物群的元素似乎通过蛋清传递到胚胎，并构成了胚胎肠道细菌种群的大部分。
