Laboratoire de Biochimie et de Génie Enzymatique des Lipases, ENIS, Route de Soukra, Sfax, Tunisie.
Laboratoire de Génie Enzymatique et de Microbiologie, Ecole Nationale d'Ingénieurs de Sfax, Sfax, Tunisia.
Biotechnol Prog. 2019 Jul;35(4):e2833. doi: 10.1002/btpr.2833. Epub 2019 May 13.
A mesophilic bacterial culture, producing an extracellular alkaline lipase, was isolated from the gas-washing wastewaters generated from the Sfax phosphate plant of the Tunisian Chemical Group and identified as Staphylococcus capitis strain. The lipase, named S. capitis lipase (SCL), has been purified to homogeneity from the culture medium. The purified enzyme molecular weight was around 45 kDa. Specific activities about 3,900 and 500 U/mg were measured using tributyrin and olive oil emulsion as substrates, respectively at 37°C and pH 8.5. Interestingly, the SCL maintained more than 60% of its initial activity over a wide pH values ranging from 5 to 11 with a high stability between pH 9 and 11 after 1 hr of incubation at room temperature. The lipase activity was enhanced in the presence of 2 mM of Mg , Ca , and K . SCL showed significant stability in the presence of detergents and organic solvents. Altogether, these features make the SCL useful for industrial applications. Besides, SCL was compatible with commercially available detergents, and its incorporation increases lipid degradation performances making it a potential candidate in detergent formulation.
从突尼斯化学品集团的斯法克斯磷酸盐厂产生的气体洗涤废水中分离出一种产生细胞外碱性脂肪酶的嗜温细菌培养物，并将其鉴定为葡萄球菌属菌株。这种脂肪酶被命名为 S. capitis 脂肪酶 (SCL)，已从培养基中纯化为均相。纯化酶的分子量约为 45 kDa。在 37°C 和 pH 8.5 下，使用三丁酸甘油酯和橄榄油乳液作为底物，分别测量到约 3900 和 500 U/mg 的比活性。有趣的是，SCL 在 pH 值为 5 到 11 的宽范围内保持超过 60%的初始活性，并且在室温下孵育 1 小时后在 pH 值为 9 到 11 之间具有很高的稳定性。在存在 2 mM 的 Mg、Ca 和 K 的情况下，脂肪酶活性增强。SCL 在存在洗涤剂和有机溶剂时表现出显著的稳定性。总的来说，这些特性使 SCL 可用于工业应用。此外，SCL 与市售的洗涤剂兼容，其掺入可提高脂质降解性能，使其成为洗涤剂配方中的潜在候选物。
