• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码 RNA TCTN2 的过表达通过增强细胞自噬来保护脊髓损伤中的神经元免于凋亡。

Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury.

机构信息

Department of Rehabilitation, The General Hospital, Tianjin Medical University, China.

出版信息

FEBS Open Bio. 2019 Jul;9(7):1223-1231. doi: 10.1002/2211-5463.12651. Epub 2019 Jun 4.

DOI:10.1002/2211-5463.12651
PMID:31050183
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6609579/
Abstract

Neuronal apoptosis is the main pathological feature of spinal cord injury (SCI), while autophagy contributes to ameliorating neuronal damage via inhibition of apoptosis. Here, we investigated the role of tectonic family member 2 (TCTN2) long non-coding RNA on apoptosis and autophagy in SCI. TCTN2 was down-regulated in the spinal cord tissues of a rat model of SCI and in oxygen-glucose deprivation-induced hypoxic SY-SH-5Y cells, while microRNA-216b (miR-216b) was up-regulated. Overexpression of TCTN2 reduced neuron apoptosis by inducing autophagy, and TCTN2 was observed to negatively regulate miR-216b. Furthermore, TCTN2 promoted autophagy to repress apoptosis through the miR-216b-Beclin-1 pathway, and overexpression of TCTN2 improved neurological function in the SCI rat model. In summary, our data suggest that TCTN2 enhances autophagy by targeting the miR-216b-Beclin-1 pathway, thereby ameliorating neuronal apoptosis and relieving spinal cord injury.

摘要

神经元凋亡是脊髓损伤 (SCI) 的主要病理特征，而自噬通过抑制细胞凋亡有助于减轻神经元损伤。在这里，我们研究了结构域家族成员 2 (TCTN2) 长非编码 RNA 在 SCI 中对细胞凋亡和自噬的作用。在 SCI 大鼠模型和氧葡萄糖剥夺诱导的缺氧 SY-SH-5Y 细胞中，TCTN2 表达下调，而 microRNA-216b (miR-216b) 表达上调。TCTN2 的过表达通过诱导自噬减少神经元凋亡，并且观察到 TCTN2 负调控 miR-216b。此外，TCTN2 通过 miR-216b-Beclin-1 通路促进自噬来抑制细胞凋亡，并且 TCTN2 的过表达改善了 SCI 大鼠模型的神经功能。总之，我们的数据表明，TCTN2 通过靶向 miR-216b-Beclin-1 通路增强自噬，从而减轻神经元凋亡并缓解脊髓损伤。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/52d5d3d20a72/FEB4-9-1223-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/03398914bb52/FEB4-9-1223-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/3c143a1f13a6/FEB4-9-1223-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/49e8ab04c53f/FEB4-9-1223-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/9b710ada1a44/FEB4-9-1223-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/52d5d3d20a72/FEB4-9-1223-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/03398914bb52/FEB4-9-1223-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/3c143a1f13a6/FEB4-9-1223-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/49e8ab04c53f/FEB4-9-1223-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/9b710ada1a44/FEB4-9-1223-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2fb4/6609579/52d5d3d20a72/FEB4-9-1223-g005.jpg

相似文献

1
Overexpression of lncRNA TCTN2 protects neurons from apoptosis by enhancing cell autophagy in spinal cord injury.长链非编码 RNA TCTN2 的过表达通过增强细胞自噬来保护脊髓损伤中的神经元免于凋亡。
FEBS Open Bio. 2019 Jul;9(7):1223-1231. doi: 10.1002/2211-5463.12651. Epub 2019 Jun 4.
2
Exosomes Derived from lncRNA TCTN2-Modified Mesenchymal Stem Cells Improve Spinal Cord Injury by miR-329-3p/IGF1R Axis.来源于长链非编码 RNA TCTN2 修饰的间充质干细胞的外泌体通过 miR-329-3p/IGF1R 轴改善脊髓损伤。
J Mol Neurosci. 2022 Mar;72(3):482-495. doi: 10.1007/s12031-021-01914-7. Epub 2021 Oct 8.
3
RETRACTED: Long Coding RNA XIST Contributes to Neuronal Apoptosis through the Downregulation of AKT Phosphorylation and Is Negatively Regulated by miR-494 in Rat Spinal Cord Injury.撤回：长链编码RNA XIST通过下调AKT磷酸化促进神经元凋亡，并在大鼠脊髓损伤中受到miR-494的负调控。
Int J Mol Sci. 2017 Apr 1;18(4):732. doi: 10.3390/ijms18040732.
4
Hydrogen sulfide upregulated lncRNA CasC7 to reduce neuronal cell apoptosis in spinal cord ischemia-reperfusion injury rat.硫化氢上调长链非编码 RNA CasC7 减少脊髓缺血再灌注损伤大鼠神经元细胞凋亡。
Biomed Pharmacother. 2018 Feb;98:856-862. doi: 10.1016/j.biopha.2017.12.079. Epub 2018 Jan 6.
5
Overexpression of long non-coding RNA LINC00158 inhibits neuronal apoptosis by promoting autophagy in spinal cord injury.长链非编码RNA LINC00158的过表达通过促进脊髓损伤中的自噬来抑制神经元凋亡。
Chin J Physiol. 2022 Nov-Dec;65(6):282-289. doi: 10.4103/0304-4920.360035.
6
Beclin-1-mediated autophagy protects spinal cord neurons against mechanical injury-induced apoptosis.贝林1介导的自噬保护脊髓神经元免受机械损伤诱导的凋亡。
Apoptosis. 2014 Jun;19(6):933-45. doi: 10.1007/s10495-014-0976-1.
7
Knockdown of miR-372 Inhibits Nerve Cell Apoptosis Induced by Spinal Cord Ischemia/Reperfusion Injury via Enhancing Autophagy by Up-regulating Beclin-1.敲低 miR-372 通过上调 Beclin-1 增强自噬抑制脊髓缺血再灌注损伤诱导的神经细胞凋亡。
J Mol Neurosci. 2018 Nov;66(3):437-444. doi: 10.1007/s12031-018-1179-y. Epub 2018 Oct 9.
8
The PVT1/miR-216b/Beclin-1 regulates cisplatin sensitivity of NSCLC cells via modulating autophagy and apoptosis.PVT1/miR-216b/Beclin-1 通过调节自噬和凋亡调控 NSCLC 细胞对顺铂的敏感性。
Cancer Chemother Pharmacol. 2019 May;83(5):921-931. doi: 10.1007/s00280-019-03808-3. Epub 2019 Mar 11.
9
Exendin-4 Enhances Motor Function Recovery via Promotion of Autophagy and Inhibition of Neuronal Apoptosis After Spinal Cord Injury in Rats.Exendin-4 通过促进自噬和抑制脊髓损伤后神经元凋亡来增强大鼠的运动功能恢复。
Mol Neurobiol. 2016 Aug;53(6):4073-4082. doi: 10.1007/s12035-015-9327-7. Epub 2015 Jul 22.
10
Knockdown of lncRNA BDNF-AS suppresses neuronal cell apoptosis via downregulating miR-130b-5p target gene PRDM5 in acute spinal cord injury.lncRNA BDNF-AS 敲低通过下调 miR-130b-5p 靶基因 PRDM5 抑制急性脊髓损伤中的神经元细胞凋亡。
RNA Biol. 2018;15(8):1071-1080. doi: 10.1080/15476286.2018.1493333. Epub 2018 Sep 10.

引用本文的文献

1
LncRNA MIR155HG suppresses cell apoptosis by activating autophagy via miR-7036b-3p/GPNMB axis and AMPK/mTOR signaling in spinal cord injury.长链非编码RNA MIR155HG通过miR-7036b-3p/GPNMB轴和AMPK/mTOR信号通路激活自噬，从而抑制脊髓损伤中的细胞凋亡。
Noncoding RNA Res. 2025 May 22;15:1-17. doi: 10.1016/j.ncrna.2025.05.013. eCollection 2025 Dec.
2
LncRNAs Orchestrating Neuroinflammation: A Comprehensive Review.长链非编码RNA对神经炎症的调控：综述
Cell Mol Neurobiol. 2025 Mar 8;45(1):21. doi: 10.1007/s10571-025-01538-0.
3
Targeted Therapy of Spinal Cord Injury: Inhibition of Apoptosis Is a Promising Therapeutic Strategy.

本文引用的文献

1
Downregulation of long noncoding RNA Sox2ot protects PC-12 cells from hydrogen peroxide-induced injury in spinal cord injury via regulating the miR-211-myeloid cell leukemia-1 isoform2 axis.长链非编码 RNA Sox2ot 的下调通过调节 miR-211-髓样细胞白血病-1 异构体 2 轴保护 PC-12 细胞免受脊髓损伤中的过氧化氢诱导的损伤。
J Cell Biochem. 2018 Dec;119(12):9675-9684. doi: 10.1002/jcb.27280. Epub 2018 Aug 26.
2
LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation.长链非编码RNA MALAT1通过调控miR-204在大鼠脊髓缺血再灌注损伤模型中发挥神经保护作用。
Curr Neurovasc Res. 2018;15(3):211-219. doi: 10.2174/1567202615666180712153150.
3
脊髓损伤的靶向治疗：抑制细胞凋亡是一种很有前途的治疗策略。
Mol Neurobiol. 2024 Jul;61(7):4222-4239. doi: 10.1007/s12035-023-03814-w. Epub 2023 Dec 8.
4
Tanshinone IIA protects motor neuron-like NSC-34 cells against lipopolysaccharide-induced cell injury by the regulation of the lncRNA TCTN2/miR-125a-5p/DUSP1 axis.丹参酮IIA通过调控lncRNA TCTN2/miR-125a-5p/DUSP1轴保护运动神经元样NSC-34细胞免受脂多糖诱导的细胞损伤。
Regen Ther. 2023 Sep 13;24:417-425. doi: 10.1016/j.reth.2023.03.007. eCollection 2023 Dec.
5
Research Progress of Antioxidants in Oxidative Stress Therapy after Spinal Cord Injury.脊髓损伤后氧化应激治疗中抗氧化剂的研究进展
Neurochem Res. 2023 Dec;48(12):3473-3484. doi: 10.1007/s11064-023-03993-x. Epub 2023 Aug 1.
6
Neuroprotective Effects of Oxymatrine via Triggering Autophagy and Inhibiting Apoptosis Following Spinal Cord Injury in Rats.氧化苦参碱通过诱导自噬和抑制大鼠脊髓损伤后的细胞凋亡发挥神经保护作用。
Mol Neurobiol. 2023 Aug;60(8):4450-4471. doi: 10.1007/s12035-023-03364-1. Epub 2023 Apr 28.
7
Protective Effect of Hydrogen-Rich Saline on Spinal Cord Damage in Rats.富氢盐水对大鼠脊髓损伤的保护作用
Pharmaceuticals (Basel). 2023 Apr 1;16(4):527. doi: 10.3390/ph16040527.
8
Advances in molecular therapies for targeting pathophysiology in spinal cord injury.脊髓损伤病理生理学靶向的分子治疗进展。
Expert Opin Ther Targets. 2023 Mar;27(3):171-187. doi: 10.1080/14728222.2023.2194532. Epub 2023 Apr 5.
9
Crosstalk between exosomes and autophagy in spinal cord injury: fresh positive target for therapeutic application.外泌体与脊髓损伤自噬的相互作用：治疗应用的新靶点。
Cell Tissue Res. 2023 Jan;391(1):1-17. doi: 10.1007/s00441-022-03699-6. Epub 2022 Nov 16.
10
Regulation of Oxidative Stress by Long Non-coding RNAs in Central Nervous System Disorders.长链非编码RNA对中枢神经系统疾病中氧化应激的调控
Front Mol Neurosci. 2022 Jun 15;15:931704. doi: 10.3389/fnmol.2022.931704. eCollection 2022.
Knockdown of lncRNA BDNF-AS suppresses neuronal cell apoptosis via downregulating miR-130b-5p target gene PRDM5 in acute spinal cord injury.
lncRNA BDNF-AS 敲低通过下调 miR-130b-5p 靶基因 PRDM5 抑制急性脊髓损伤中的神经元细胞凋亡。
RNA Biol. 2018;15(8):1071-1080. doi: 10.1080/15476286.2018.1493333. Epub 2018 Sep 10.
4
Metformin Protects Against Spinal Cord Injury by Regulating Autophagy via the mTOR Signaling Pathway.二甲双胍通过调控 mTOR 信号通路抑制自噬对脊髓损伤发挥保护作用。
Neurochem Res. 2018 May;43(5):1111-1117. doi: 10.1007/s11064-018-2525-8. Epub 2018 May 4.
5
Loss of Tctn3 causes neuronal apoptosis and neural tube defects in mice.Tctn3 缺失导致小鼠神经元凋亡和神经管缺陷。
Cell Death Dis. 2018 May 1;9(5):520. doi: 10.1038/s41419-018-0563-4.
6
MiR-216b inhibits cell proliferation by targeting FOXM1 in cervical cancer cells and is associated with better prognosis.miR-216b 通过靶向作用于宫颈癌细胞中的 FOXM1 抑制细胞增殖，与较好的预后相关。
BMC Cancer. 2017 Oct 4;17(1):673. doi: 10.1186/s12885-017-3650-5.
7
RETRACTED: Long Coding RNA XIST Contributes to Neuronal Apoptosis through the Downregulation of AKT Phosphorylation and Is Negatively Regulated by miR-494 in Rat Spinal Cord Injury.撤回：长链编码RNA XIST通过下调AKT磷酸化促进神经元凋亡，并在大鼠脊髓损伤中受到miR-494的负调控。
Int J Mol Sci. 2017 Apr 1;18(4):732. doi: 10.3390/ijms18040732.
8
LncRNA MALAT-1 Elevates HMGB1 to Promote Autophagy Resulting in Inhibition of Tumor Cell Apoptosis in Multiple Myeloma.长链非编码RNA MALAT-1上调高迁移率族蛋白B1以促进自噬，从而抑制多发性骨髓瘤肿瘤细胞凋亡。
J Cell Biochem. 2017 Oct;118(10):3341-3348. doi: 10.1002/jcb.25987. Epub 2017 May 3.
9
The HIF-2α-MALAT1-miR-216b axis regulates multi-drug resistance of hepatocellular carcinoma cells via modulating autophagy.HIF-2α-MALAT1-miR-216b轴通过调节自噬来调控肝癌细胞的多药耐药性。
Biochem Biophys Res Commun. 2016 Sep 23;478(3):1067-73. doi: 10.1016/j.bbrc.2016.08.065. Epub 2016 Aug 11.
10
Loss of autophagy-related protein Beclin 1 may define poor prognosis in ovarian clear cell carcinomas.自噬相关蛋白Beclin 1的缺失可能预示卵巢透明细胞癌的预后不良。
Int J Oncol. 2015 Dec;47(6):2037-44. doi: 10.3892/ijo.2015.3191. Epub 2015 Oct 6.