• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

长链非编码RNA MIR155HG通过miR-7036b-3p/GPNMB轴和AMPK/mTOR信号通路激活自噬，从而抑制脊髓损伤中的细胞凋亡。

LncRNA MIR155HG suppresses cell apoptosis by activating autophagy via miR-7036b-3p/GPNMB axis and AMPK/mTOR signaling in spinal cord injury.

作者信息

Liu Ruoxi, Ye Jintao, Huang Sihua

机构信息

Department of Orthopaedic Surgery, Second Affiliated Hospital of Medical School of Xi'an Jiaotong University, Xi'an, 710004, China.

出版信息

Noncoding RNA Res. 2025 May 22;15:1-17. doi: 10.1016/j.ncrna.2025.05.013. eCollection 2025 Dec.

DOI:10.1016/j.ncrna.2025.05.013
PMID:40703249
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12281537/
Abstract

BACKGROUND

Long non-coding RNAs (lncRNAs) participate in spinal cord injury (SCI) development through regulating autophagy and neuronal apoptosis. Previously, was identified as an upregulated lncRNA in rat bladder tissues harvested after SCI operation. Our study aimed to elucidate the function of in SCI.

METHODS

Glutamate (Glu)-stimulated primary mouse spinal cord neurons were used as SCI cellular models. Contusion-induced SCI mouse models were established using an improved weightlessness method. Neuronal apoptosis and autophagy affected by or silencing were assessed by TUNEL staining, flow cytometry assay, western blotting, and immunofluorescence staining. The binding of miR-7036b-3p on (or ) was verified by luciferase reporter assay. Histological changes were observed through HE and Masson staining.

RESULTS

and expression was elevated while miR-7036b-3p expression was reduced in SCI. silencing attenuated the apoptosis in Glu-stimulated neurons and ameliorated glial scar formation and motor function of SCI mice. knockdown mitigated apoptosis, enhanced autophagy, activated AMPK phosphorylation, and repressed mTOR phosphorylation. upregulated expression by sponging miR-7036b-3p. The autophagy inhibitor 3-MA reversed the above changes caused by depletion.

CONCLUSION

knockdown alleviated neuronal apoptosis by enhancing autophagy in SCI via miR-7036b-3p/ axis and AMPK/mTOR pathway.

摘要

背景

长链非编码RNA（lncRNAs）通过调节自噬和神经元凋亡参与脊髓损伤（SCI）的发展。此前，已被鉴定为脊髓损伤手术后大鼠膀胱组织中上调的lncRNA。我们的研究旨在阐明在脊髓损伤中的作用。

方法

将谷氨酸（Glu）刺激的原代小鼠脊髓神经元用作脊髓损伤的细胞模型。采用改良的失重法建立挫伤性脊髓损伤小鼠模型。通过TUNEL染色、流式细胞术检测、蛋白质印迹法和免疫荧光染色评估受或沉默影响的神经元凋亡和自噬。通过荧光素酶报告基因检测验证miR-7036b-3p与（或）的结合。通过苏木精-伊红（HE）和Masson染色观察组织学变化。

结果

在脊髓损伤中，和的表达升高，而miR-7036b-3p的表达降低。沉默可减轻Glu刺激的神经元中的细胞凋亡，并改善脊髓损伤小鼠的胶质瘢痕形成和运动功能。敲低可减轻细胞凋亡，增强自噬，激活AMPK磷酸化，并抑制mTOR磷酸化。通过海绵吸附miR-7036b-3p上调的表达。自噬抑制剂3-MA可逆转耗竭引起的上述变化。

结论

敲低通过miR-7036b-3p/轴和AMPK/mTOR途径增强脊髓损伤中的自噬，从而减轻神经元凋亡。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/f89b8eb7d9b6/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/a2f3fd4bbc46/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/625f610308df/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/d7cd393509f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/62c15d904a9a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/b1598b31facf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/1a0ec706102f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/2471d965d39a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/15b737044454/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/d122a821a2aa/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/f7f1890681b1/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/6a4865489542/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/f89b8eb7d9b6/gr11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/a2f3fd4bbc46/ga1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/625f610308df/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/d7cd393509f8/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/62c15d904a9a/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/b1598b31facf/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/1a0ec706102f/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/2471d965d39a/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/15b737044454/gr7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/d122a821a2aa/gr8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/f7f1890681b1/gr9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/6a4865489542/gr10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d43/12281537/f89b8eb7d9b6/gr11.jpg

相似文献

1
LncRNA MIR155HG suppresses cell apoptosis by activating autophagy via miR-7036b-3p/GPNMB axis and AMPK/mTOR signaling in spinal cord injury.长链非编码RNA MIR155HG通过miR-7036b-3p/GPNMB轴和AMPK/mTOR信号通路激活自噬，从而抑制脊髓损伤中的细胞凋亡。
Noncoding RNA Res. 2025 May 22;15:1-17. doi: 10.1016/j.ncrna.2025.05.013. eCollection 2025 Dec.
2
miR-210 Regulates Autophagy Through the AMPK/mTOR Signaling Pathway, Reduces Neuronal Cell Death and Inflammatory Responses, and Enhances Functional Recovery Following Cerebral Hemorrhage in Mice.微小RNA-210通过AMPK/雷帕霉素靶蛋白信号通路调节自噬，减少神经元细胞死亡和炎症反应，并增强小鼠脑出血后的功能恢复。
Neurochem Res. 2025 Jun 5;50(3):180. doi: 10.1007/s11064-025-04434-7.
3
Blocking the MIR155HG/miR-155 axis reduces CTGF-induced inflammatory cytokine production and α-SMA expression via upregulating AZGP1 in hypertrophic scar fibroblasts.阻断 MIR155HG/miR-155 轴通过上调增殖细胞核抗原在肥厚性瘢痕成纤维细胞中减少 CTGF 诱导的炎症细胞因子产生和 α-SMA 表达。
Cell Signal. 2024 Aug;120:111202. doi: 10.1016/j.cellsig.2024.111202. Epub 2024 May 9.
4
LncRNA XIST promotes carboplatin resistance of ovarian cancer through activating autophagy via targeting miR-506-3p/FOXP1 axis.长链非编码 RNA XIST 通过靶向 miR-506-3p/FOXP1 轴激活自噬促进卵巢癌细胞对卡铂的耐药性。
J Gynecol Oncol. 2022 Nov;33(6):e81. doi: 10.3802/jgo.2022.33.e81.
5
Long non-coding RNA HOXA11-AS inhibits apoptosis, induces proliferation, and promotes autophagy via the miR-214-3p/ATG12 axis in acute T lymphoblastic leukemia.长链非编码RNA HOXA11-AS通过miR-214-3p/ATG12轴抑制急性T淋巴细胞白血病细胞凋亡、诱导增殖并促进自噬。
J Mol Histol. 2025 May 26;56(3):170. doi: 10.1007/s10735-025-10462-y.
6
miR-29c-3p downregulation accelerates spinal cord injury progression by targeting BRD4.miR-29c-3p下调通过靶向BRD4加速脊髓损伤进展。
J Orthop Surg Res. 2025 Jul 25;20(1):703. doi: 10.1186/s13018-025-06108-0.
7
MiR-24-3p modulates cardiac function in doxorubicin -induced heart failure via the Sp1/PI3K signaling pathway.miR-24-3p 通过 Sp1/PI3K 信号通路调节阿霉素诱导的心力衰竭中的心脏功能。
Cell Signal. 2024 Dec;124:111407. doi: 10.1016/j.cellsig.2024.111407. Epub 2024 Sep 14.
8
Expression of Concern.关注声明。
Eur Rev Med Pharmacol Sci. 2025 Jun;29(6):287-288. doi: 10.26355/eurrev_202506_37270.
9
Exosomal miR-17-92 Cluster from BMSCs Alleviates Apoptosis and Inflammation in Spinal Cord Injury.骨髓间充质干细胞来源的外泌体miR-17-92簇减轻脊髓损伤中的细胞凋亡和炎症
Biochem Genet. 2024 Jul 3. doi: 10.1007/s10528-024-10876-5.
10
MIR155HG promotes metastasis and cisplatin resistance of cervical cancer cells by regulating the miR-409-3p and ZEB1 axis.MIR155HG通过调控miR-409-3p和ZEB1轴促进宫颈癌细胞的转移和顺铂耐药。
Sci Rep. 2025 Jul 1;15(1):20541. doi: 10.1038/s41598-025-08727-3.

本文引用的文献

1
Mir155hg Accelerates Hippocampal Neuron Injury in Convulsive Status Epilepticus by Inhibiting Microglial Phagocytosis.Mir155hg通过抑制小胶质细胞吞噬作用加速惊厥性癫痫持续状态下的海马神经元损伤。
Neurochem Res. 2024 Jul;49(7):1782-1793. doi: 10.1007/s11064-024-04131-x. Epub 2024 Mar 30.
2
Gpnmb silencing protects against hyperoxia-induced acute lung injury by inhibition of mitochondrial-mediated apoptosis.Gpnmb 沉默通过抑制线粒体介导的细胞凋亡来防止高氧诱导的急性肺损伤。
Hum Exp Toxicol. 2024 Jan-Dec;43:9603271231222873. doi: 10.1177/09603271231222873.
3
Neuroprotective Effects of Oxymatrine via Triggering Autophagy and Inhibiting Apoptosis Following Spinal Cord Injury in Rats.
氧化苦参碱通过诱导自噬和抑制大鼠脊髓损伤后的细胞凋亡发挥神经保护作用。
Mol Neurobiol. 2023 Aug;60(8):4450-4471. doi: 10.1007/s12035-023-03364-1. Epub 2023 Apr 28.
4
The Importance of mTORC1-Autophagy Axis for Skeletal Muscle Diseases.mTORC1-自噬轴在肌肉骨骼疾病中的重要性。
Int J Mol Sci. 2022 Dec 24;24(1):297. doi: 10.3390/ijms24010297.
5
Overexpression of long non-coding RNA LINC00158 inhibits neuronal apoptosis by promoting autophagy in spinal cord injury.长链非编码RNA LINC00158的过表达通过促进脊髓损伤中的自噬来抑制神经元凋亡。
Chin J Physiol. 2022 Nov-Dec;65(6):282-289. doi: 10.4103/0304-4920.360035.
6
HSPA1A ameliorated spinal cord injury in rats by inhibiting apoptosis to exert neuroprotective effects.热休克蛋白 70 家族成员 1A 通过抑制细胞凋亡发挥神经保护作用，改善大鼠脊髓损伤。
Exp Neurol. 2023 Mar;361:114301. doi: 10.1016/j.expneurol.2022.114301. Epub 2022 Dec 17.
7
The Role of Autophagy in Childhood Central Nervous System Tumors.自噬在儿童中枢神经系统肿瘤中的作用。
Curr Treat Options Oncol. 2022 Nov;23(11):1535-1547. doi: 10.1007/s11864-022-01015-6. Epub 2022 Oct 5.
8
Cytoskeletal vimentin regulates cell size and autophagy through mTORC1 signaling.细胞骨架中间丝波形蛋白通过 mTORC1 信号调节细胞大小和自噬。
PLoS Biol. 2022 Sep 13;20(9):e3001737. doi: 10.1371/journal.pbio.3001737. eCollection 2022 Sep.
9
GPNMB: a potent inducer of immunosuppression in cancer.GPNMB：癌症中具有强大免疫抑制作用的诱导剂。
Oncogene. 2022 Oct;41(41):4573-4590. doi: 10.1038/s41388-022-02443-2. Epub 2022 Sep 1.
10
GPNMB confers risk for Parkinson's disease through interaction with α-synuclein.GPNMB 通过与 α-突触核蛋白相互作用，增加帕金森病发病风险。
Science. 2022 Aug 19;377(6608):eabk0637. doi: 10.1126/science.abk0637.