Vargis Vidhu Sara, Jayachandran Priya Chandhana, Surendran Harsha, Punathil Vasu Suneesh, Nair Bipin, Thekkedath Gopalakrishnan Satheesh Babu
Department of Sciences, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, Tamil Nadu, India.
International School of Photonics, Cochin University of Science and Technology, Cochin, Kerala, India.
IET Nanobiotechnol. 2019 Apr;13(2):107-113. doi: 10.1049/iet-nbt.2018.5150.
This study describes the development and testing of a simple and novel enzyme-free nanolabel for the detection and signal amplification in a sandwich immunoassay. Gold nanoparticles decorated reduced graphene oxide (rGOAu) was used as the nanolabel for the quantitative detection of human immunoglobulin G (HIgG). The rGOAu nanolabel was synthesised by one pot chemical reduction of graphene oxide and chloroauric acid using sodium borohydride. The pseudo-peroxidase behaviour of rGOAu makes the nanolabel unique from other existing labels. The immunosensing platform was fabricated using self-assembled monolayers of 11-mercaptoundecanoic acid (11-MUDA) on a gold disc electrode. The covalent immobilisation of antibody was achieved through the bonding of the carboxyl group of 11-MUDA and the amino group of the antibody using chemical linkers [1-ethyl-3-(3-dimethylaminopropyl)carbodiimide] and -hydroxysuccinimide. The fabricated immunosensor exhibited a linear range that included HIgG concentrations of 62.5-500 ng ml. The sensor was also used for the testing of HIgG in the blood sample.
本研究描述了一种简单新颖的无酶纳米标记物的开发与测试，用于夹心免疫分析中的检测和信号放大。用金纳米粒子修饰的还原氧化石墨烯（rGOAu）作为纳米标记物，用于定量检测人免疫球蛋白G（HIgG）。rGOAu纳米标记物是通过用硼氢化钠对氧化石墨烯和氯金酸进行一锅法化学还原合成的。rGOAu的拟过氧化物酶行为使其与其他现有标记物不同。免疫传感平台是在金盘电极上使用11-巯基十一烷酸（11-MUDA）的自组装单分子层构建的。通过使用化学交联剂[1-乙基-3-（3-二甲基氨基丙基）碳二亚胺]和N-羟基琥珀酰亚胺，使11-MUDA的羧基与抗体的氨基结合，实现抗体的共价固定。所构建的免疫传感器的线性范围包括62.5-500 ng/ml的HIgG浓度。该传感器还用于检测血样中的HIgG。
